摘要
目的:采用星点设计-效应面法优化复方巴戟天生骨颗粒的提取工艺。方法:按试验设计制备复方巴戟天生骨颗粒,以处方药材提取浓缩液中的淫羊藿苷含量、干膏率的综合评分OD为指标,分别对药材浸泡时间、料液比、提取时间、减压浓缩温度进行单因素考察,在此基础上选择对响应值有显著影响的料液比、提取时间、减压浓缩温度3个因素采取三因素五水平星点设计,考察其对该制剂提取效果的影响。结果:复方巴戟天生骨颗粒的最佳提取工艺为:料液比1∶11.33、提取时间39 min、减压浓缩温度66℃。成品的淫羊藿苷含量和干膏率与预测值的偏差分别为1.60%和1.55%。结论:星点设计-效应面法适用于复方巴戟天生骨颗粒工艺优化,该方法建立的数学模型预测性良好。
Objective： To optimize extraction process of Fufang Bajitian Shenggu Particles by central composite design and response surface method. Methods： The preparation of Fufang Bajitian Shenggu Particles was designed according to the test. With the overall desirability（OD） of lcariin content and the dry extract yield in medicinal materials extract concentrate as examining indexes,immersion time,material liquid ratio,extraction time and stress concentration temperature were investigated. Based on the single factor tests,material liquid ratio,extraction time and stress concentration temperature which impacted response values significantly were investigated by three factors and five levels of central composite design. Results： Optimum extraction technology of Fufang Bajitian Shenggu Particles were as follows： material liquid ratio was 1∶ 11. 33,extraction time was 39 min,and stress concentration temperature was 66 ℃. Bias of the lcariin content and the dry extract yield between observed and predicted values were 1. 60% and 1. 55%,respectively. Conclusions：Using central composite design and response surface method to optimize extraction of Fufang Bajitian Shenggu particles has a good prediction.
出处
《中药材》
CAS
CSCD
北大核心
2014年第10期1863-1867,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81173283)
关键词
复方巴戟天生骨颗粒
综合评分
提取工艺
星点设计
效应面法
Fufang Bajitian Shenggu Particles
Overall desirability
Extraction technology
Central composite design
Response surface method
