摘要
目的 :研究脑溢安 (NYA)颗粒剂对出血性中风大鼠脑内HSP70与NF kB表达的影响。方法 :用Ⅶ型胶原酶立体定位法复制出血性中风动物模型 ,观察行为、TUNEL及HE染色 ,HSP70、NF kB免疫组化反应 ,取手术侧海马及皮质分别抽提总蛋白与核蛋白进行Westernblot反应 ,密度扫描测定HSP70与NF kB蛋白表达。结果 :NYA治疗组爬行计分明显低于模型对照组 ,3d时血肿明显缩小 ,7d基本吸收 ,凋亡细胞明显减小 ;免疫组化示 3d时可见 ,HSP70信号增强 ,NF kB信号相对减弱 ;Westernblot及密度扫描显示HSP70显著升高 ,NF kB显著降低。结论 :NYA能促进神经功能恢复 ,加快血肿吸收 ;增强HSP70表达 ,抑制NF kB表达 ,通过抑制炎症因子保护脑出血后继发脑缺血损伤可能是NYA的作用机理之一。
Objective:To observe the effects of Traditional Chinese Medicine Nao Yi An Granule on the expression of HSP70 and NF kB in rat brain after experimental haemorrhagic stroke.Methods:Haemorrhagic stroke rat model was made by injecting collagenase type Ⅶ stereotaxically,and was taken for Behavior observation;TUNEL and HE staining,HSP70 and NF kB immunohistochemistry;cortex of operation side were taken,then total protein and nuclear protein were extracted respectively for western blot analysis,density scanning was done to detect the protein content of HSP70 and NF kB to study the effect of NYA on them. Results:Climbing scores showed that NYA group was significantly lower than model control group;Light microscope study showed that hematoma of NYA group were obviously diminished 3 days after operation and were almost absorbed in 7 days,TUNEL showed that apoptosis cells were decreased in NYA group,immunohistochemistry showed that HSP70 and NF kB was expressed extensively in rat brain.HSP70 signals were increased and NF kB signals were decreased 3 days after NYA treatment. Western blot and density scanning showed that HAP70 expression was significantly increased and NF kB was significantly decreased 3 and 7 days after operation in NYA group. Conclusion:NYA promotes the recovery of neurological deficits,increases the hematoma absorption;enhances HSP70 expression and inhibits NF kB expression and this can be one of very important mechanism of NYA.
出处
《中国中医基础医学杂志》
CAS
CSCD
2002年第6期20-23,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家中医药管理局项目 (No .2 0 0 0 J B 0 3 )
