摘要
根据已报道的HSP基因序列(EMB登录号:AF506290),设计出特异引物,PCR扩增后测序,获得1.7 kb大小的大黄鱼热激蛋白cDNA基因序列。HSP氨基酸序列的系统进化分析表明:不同物种编码的HSP存在一些明显相关的群体,高等动物编码的HSP在进化上紧密相关,形成一个显著群体,大黄鱼编码的HSP蛋白位于高等动物群体中,这与大黄鱼的亲缘关系大致吻合,其中与非洲爪蟾的同源性最高(92.3%),也间接印证了脊椎动物中两栖纲动物与鱼纲动物的亲源关系。通过大黄鱼HSP基因的提取和分析,可为以后制备出核酸探针、筛选和克隆出一批具有优良性状的基因、构建基因文库等研究工作奠定基础,同时对大黄鱼的品质改良和良种的选育有重要意义。
Divergent primers were designed for HSP amplification according to the sequence in Genbank （EMB ： AF506290）. After PCR and sequencing, it was showed that the HSP gene sequence of P. crocea was about 1.7 kb in length. According to the phylogenic tree, the tree consisted of some distinct branches, which were constructed by HSP of different species, and animals belonged to the same branch. And P. crocea shared highest homology with Venous leaves （African clawed frog）, which confirmed the hypothesis that the Amphibian was subsequent to the Pisces in the evolutionary lineage partially. And the construction work in this paper could lay a basis for subsequent work of nucleotide probe preparation, clone and selection of some kinds of fine functional genes for culture, construction of P. crocea cDNA library, and was of great meaning for the development for the culture of P. crocea.
出处
《上海水产大学学报》
CAS
CSCD
北大核心
2007年第2期103-108,共6页
Journal of Shanghai Fisheries University
基金
国家高技术研究发展计划("863")项目(2002AA603021)
宁波市重大招标项目(2004C100040)
关键词
大黄鱼
热激蛋白cDNA
克隆
系统发育
Pseudosciaena crocea （Richardson）
heat shock protein （HSP） cDNA
clone
phylogenic analysis
