摘要
杀菌剂是影响生防菌应用的重要因素,抗药性实验是评价生防菌耐受杀菌剂作用的基础工作。本研究采用牛津杯法和平板菌落计数法,测定5种常用杀菌剂对烟草疫霉菌有拮抗作用的地衣芽孢杆菌、枯草芽孢杆菌和解淀粉芽孢杆菌3个菌株的抑菌作用。结果表明:(1)35%甲霜灵、80%代森锰锌、722 g/L霜霉威盐酸盐对3种拮抗菌无抑制作用,表明可以和供试的3种拮抗菌混配使用;(2)58%甲霜·锰锌和68%精甲霜·锰锌对3种细菌有抑制作用,甲霜·锰锌对3种细菌的EC50值平均为221.9μg/m L,精甲霜·锰锌对3种拮抗菌的EC50值平均为195.5μg/m L,表明不能和供试的3种拮抗菌混配使用。
Fungicide is the key factors to affect the application of bio-control bacteria, and the experiment of drug resistance is the basic work to estimate the influence of bio-control bacteria tolerance pesticide. In this study, five kinds of commonly fungicides were used to antagonize Bacillus licheniformis, B. subtilis and B. amyloliquefaciens with antagonistic Phytophora nicotianae by oxford cup method and plate colony counting. Results showed that： （1） 35% metalaxyl, 80% mancozeb and 722 g/L propamocarb hydrochloride had no bacteriostatic activity, which indicated they could be respectively used mixed with the Bacillus; （2） 58% metalaxyl-mancozeb and 68% metaldehyde-maneozeb had inhibition, the average EC50 of 58% metalaxyl-mancozeb reached 221.9 μg/mL, and the average ECso of 68% metaldehyde-mancozeb reached 195.5 μg/mL, indicated they can not be respectively used mixed with the bacillus.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第6期937-940,共4页
Journal of Yunnan Agricultural University:Natural Science
基金
中国烟草公司重点科技项目(110200902065)
中国烟草总公司云南省公司科计划项目(2012YN05
2010YN18
2010YN19)
关键词
烟草疫霉
芽孢杆菌
杀菌剂
抑菌实验
Phytophora parasitica
Bacillcus
fungicides
bacteriostatic test
