摘要
目的:探讨丹皮酚对血管性认知障碍(VCI)小鼠认知功能及海马神经元损伤的影响。方法:雄性C57BL/6小鼠60只,随机分为假手术(Sham)组、模型(VCI)组、丹皮酚(Pae)低、中、高剂量治疗组,每组12只。采用双侧颈总动脉结扎(2VO)制备小鼠反复缺血再灌注VCI模型,Morris水迷宫检测小鼠的认知功能,尼氏染色观察小鼠海马神经元的形态学变化,比色法检测海马SOD活性、MDA和NO含量的变化。结果:VCI小鼠认知功能下降(P<0.01),海马CA1区神经元变性坏死,MDA和NO含量增加,SOD活性降低(P<0.01);丹皮酚中、高剂量治疗可显著提高VCI小鼠的学习记忆能力,减少VCI所致的海马CA1区神经元死亡,并降低MDA和NO含量,升高SOD活性(P<0.05或P<0.01)。结论:丹皮酚可有效增强VCI小鼠的认知功能,并改善海马神经元病理改变,可能与其抗氧化应激有关。
Objective： To investigate the effects of paeonol on cognitive dysfunctions and hippocampal neuronal injury of vascular cognitive impairment （VCI） mice. Methods： Sixty C57BL/6 mice were randomly di- vided into sham opertated group, the VCI group and the paeonol treated groups （low, medium and high dose）. The VCI models were dupilicated by the bilateral common carotid artery occlusion （2VO）. The cognitive impairments were observed by Morris water maze. The contents of MDA, SOD and NO in hippocampus were determined spectrometrically. The hippocampal neuron injuries in the CA1 region were detected by nissle staining. Results： VCI induced the decreased cognitive function and neuron injury in the hippocampal CA1 region （P〈0.01）. And MDA and NO contents were significantly increased, whereas SOD activity was dramatically decreased after VCI （P〈0.01）. Compared with VCI model goup, the medium and high dose of paeonol treatment significantly im- proved cognitive impairments and reduced pathological changes in hippocampal CA1 region （P〈0.05, P〈0.01）. In additon, the medium and high dose of paeonol treatment markedly improved SOD activity, decreased MDA and NO contents in hippocampus （P〈0.05, P〈0.01）. Conclusion： Paeonol can improve cognitive impairments and neuronal injury in VCI mice, which may be related to the increased antioxidative ability of brain tissue.
出处
《温州医学院学报》
CAS
2014年第10期708-711,共4页
Journal of Wenzhou Medical College
基金
浙江省自然科学基金资助项目(Y2110679)
关键词
认知障碍
血管性
抗氧化
海马
丹皮酚
小鼠
cognitive impairment, vascular
antioxidant
hippocampus
paeonol
mice
