摘要
根据在NCBI中已登录的植物转录因子NAC家族中与抗逆性有关成员的核苷酸序列和氨基酸序列,应用生物信息学软件分析了其理化性质、疏水性/亲水性、跨膜结构、二级结构、功能结构域等,并构建了这类NAC家族成员的系统进化树。结果表明,17种NAC成员的蛋白质一级结构中存在明显的疏水区和亲水区,都存在明显的ɑ-螺旋和β折叠。二级结构组成上相似,都由ɑ-螺旋、β折叠和无规则卷曲组成。这些成员都能够通过同源建模分析NAC蛋白质的三维结构。进化分析表明,它们主要分成4大类群。通过多序列比对得到了这些成员的保守区段,并设计了简并引物。
Physicochemical property, hydrophobic/hydrophilic,membrane structure,secondary structure,functional domains of re- sistanee-realated members in the family of plant transcription factor NAC were analyzed with bioinformatics. The results showed that primary structure of 17 NAC members had obvious hydrophobic and hydrophilie areas, a-helix and 13-sheet were discovered in their primary structure prediction. Their secondary structure was composed of a-helix, 13-sheet and irregular curl. Three-dimensional structure of the NAC proteins was established by homology modeling. Phylogenetic analysis showed that they were divided into four groups. By the alignment of multiple sequence,conservative sections of these members were obtained and a pair of primer was designed. The work will pave the way for further studing the expression regulation of the NAC transcription factors related with resistance.
出处
《湖北农业科学》
北大核心
2014年第17期4199-4204,共6页
Hubei Agricultural Sciences
基金
枣庄学院博士科研基金项目(307070910)
