摘要
目的：采用 SYBR Green Ⅰ实时荧光定量 PCR 法分析肠易激综合征（ IBS）患儿肠道菌群的变化，探讨肠道相关分子微生态在 IBS发病中的作用及意义。方法根据16SrDNA 基因序列设计双歧杆菌属、乳酸杆菌属、肠球菌属、大肠杆菌、拟杆菌属、梭杆菌属、梭菌属的特异性引物，收集25份1-6岁 IBS患儿（ IBS患儿组）及25份健康儿童（健康对照组）的粪标本，采用 SYBR Green Ⅰ实时荧光定量 PCR 法测定7种细菌的数量。结果与健康对照组比较，IBS患儿组粪标本中的双歧杆菌属、乳酸杆菌属、梭杆菌属和梭菌属数量明显减少，差异有统计学意义（ P 〈0.05），大肠杆菌、肠球菌属和拟杆菌属数量无明显变化（P 〉0.05）。结论肠道菌群的变化与儿童 IBS 的发生及发展机制有一定的关系，SYBR Green I实时荧光定量 PCR技术为儿童肠道菌群组成、动态变化及相关研究提供了一种有效监测手段。
Objective To analyze the changes of intestinal microflora in children with irritable bowel syndrome （ IBS）by SYBR Green I real-time fluorescence quantitative PCR method and to explore the effect and significance of re-lated intestinal microflora on pathogenesis. Methods The specific primers of bifidobacterium,lactobacilleae,enterococ-cus,escherichia coli,bacteroides,fusobacterium and clostridium were designed according to 16SrDNA gene sequence. A total of 25 stool specimens of 1-6 years old IBS children（ IBS group ） and 25 specimens of normal controls（ control group）were collected,and then the quantities of the 7 kinds of bacteria were detected by SYBR GreenⅠ real-time PCR. Results Compared with those in control group,the quantities of bifidobacterium,lactobacilleae,fusobacterium and clostridium were significantly reduced in IBS group（ P 〈0. 05 ）,while the quantities of escherichia coli,enterococcus and bacteroides showed significant differences（P〉0. 05）. Conclusion The changes of intestinal flora in children with irritable bowel syndrome have some relationship with and incidence and development of IBS,and the SYBR Green I real-time PCR method is an effective method to monitor the construction and dynamic changes of intestinal microflora and rele-vant research.
出处
《解放军医药杂志》
CAS
2014年第9期93-96,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
关键词
肠易激综合征
儿童
肠道菌群
荧光定量
Irritable bowel syndrome
Child
Intestinal flora
Fluorescent quantification
