摘要
目的建立北刘寄奴药材中毛蕊花糖苷、木犀草素和芹菜素的含量测定方法。方法采用Kromasil C18色谱柱（4.6mm×250mm,5μm）;流动相乙腈与0.1%磷酸（22∶78）;流速1.0mL/min;柱温35℃;进样量10μL;检测波长340nm。结果毛蕊花糖苷、木犀草素和芹菜素分别在0.197 6-1.482μg、0.100 8-0.756μg和0.043 2-0.324μg范围内线性关系良好,回归方程分别为Y=7.171×10^5 X＋2.094×10^3,r=0.999 8、Y=6.833×10^5 X＋1.306×10^3,r=0.999 7、Y=7.210×10^5 X＋9.73×10^2,r=0.999 7,平均加样回收率分别为99.7%（RSD=2.0%）、99.5%（RSD=2.3%）、99.4%（RSD=2.2%）。结论所建立的方法准确性高,重复性好,可作为北刘寄奴药材的质量控制方法。
Objective To study and establish the quantitative analysis method for verbascoside,luteolin and apigenin in Siphonostegia chinensis by HPLC.Methods HPLC was performed on Kromasil C18（4.6mm×250mm,5μm）,the mobile phase consisted of acetonitrile and water（containing 0.1% phosphoric acid）at a flow rate of 1.0mL/min.The column temperature was set at 35℃,and the eluate was detected at 340 nm.Results Quantitative analysis of HPLC showed that the linear ranges of verbascoside,luteolin and apigenin were 0.197 6-1.482μg（r=0.999 8）,0.100 8-0.756μg（r=0.999 7）,0.043 2-0.324μg（r=0.999 7）respectively.The average recovery of verbascoside,luteolin and apigenin were 99.7%（RSD=2.0%）,99.5%（RSD=2.3%）,99.4%（RSD=2.2%）respectively.Conclusion The method is accurate,feasible and reliable,therefore it′s available for the quality control of Siphonostegia chinensis.
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第23期3040-3041,3044,共3页
Chongqing medicine
