摘要
目的：以绿原酸和总有机酸为研究对象，探索金银花中有效成分的纯化工艺。方法通过对比试验确定树脂型号、用量及上样流速，用正交试验法确定树脂洗脱条件，并通过试验确定萃取次数。结果经研究最优纯化工艺为：pH值为3的金银花提取液，以15 mL/min流速上样DM-101大孔树脂层析柱，1倍柱体积水洗后，以15%乙醇洗脱，洗脱速度10~15 mL/min，洗脱体积为10倍柱体积。回收乙醇洗脱液浓缩至适量，加等量乙酸乙酯萃取6次，回收乙酸乙酯，浓缩至稠膏，减压干燥，所得金银花提取物总有机酸和绿原酸含量均在85%以上。结论该法稳定、可靠，适合工业生产。
Objective To explore purification technology of effective components in Flos Lonicerace by taking chloro-genic acid and total organic acid as objects. Methods Contrast test was adopted to determine the type and amount of resin,sam-ple flow rate. Orthogonal test was applied to determine resin elution condition ,and the extraction times were determined by the experiment. Results After extraction,the optimum extraction solution of Flos Lonicerae was adjusted to PH 3,DM-101 macrop-orous resin adsorption with the wavelength rate of 15 mL/min,washing by double volume of water,using 15%ethanol elution with the rate of 10-15 mL/min and 10-fold elution volume. To the appropriate concentration after ethanol recorvery,equal amount of ethyl acetate was added and the solution was extracted 6 times,after recovery of ethyl acetate, concentrating,vacuum drying,the content of organic acids from Flos Lonicerae extracts were all more than 85%. Conclusion The method is stable,reliable,and suitable for industrial production.
出处
《现代医药卫生》
2014年第17期2584-2586,共3页
Journal of Modern Medicine & Health
关键词
工艺学
金银花
绿原酸
色谱法
高压液相
中草药
Technology
Floslonicerae
Chlorogenic acid
Chromatography,high pressure liquid
Chinese herbal
