摘要
以乙酸乙酯和0.05mol LHCl组成的混合溶液作萃取剂,从金银花中一步提取绿原酸,并研究了乙酸乙酯的体积分数、温度、浸取时间对提取绿原酸的影响和绿原酸的抗菌活性,实验结果:提取绿原酸最佳实验条件是:温度65°C,乙酸乙酯的体积分数0.8,浸取时间3h。提取物与金银花质量比为3.21%,绿原酸的质量分数86.3%;同时测得绿原酸抗金黄色葡萄球菌的活性比抗大肠杆菌强。
Chlorogenic acid was extracted from flos lonicerae by using one-step-method. The solvent was a mixed solutioin of ethyl acetate and 0.05 mol/LHCl. The effect of the volume fraction of ethyl acetate, tempeature and time on the extraction of chlorogenic acid was studied. The antimicrobial abilities of chlorogenic acid were also studied. The optimum conditions are: the volume fraction of ethyl acetate, 0.8; the temperature, 65°C;and time, 3 h. The mass ratio of the product to flos lonicerae is 3.21%, and the purity of chlorogenic acid is 86.3%. The antimicrobial abilities of chlorogenic acid against the staphylococcus aurous is better than that against Escherichia coli.
出处
《化学世界》
CAS
CSCD
北大核心
2005年第3期167-169,共3页
Chemical World
基金
湖南省教育厅自然科学基金资助项目(03C091).
关键词
金银花
乙酸乙酯
提取
绿原酸
抗菌活性
flos lonicerae
ethy acetate
extraction
chlorogenic acid
antimicrobial abilities
