摘要
建立了一种使用岛津超高效液相色谱仪LC-30A测定粮食中赭曲霉毒素A的方法.实验结果表明：在浓度范围0.550 μg/L内,校准曲线相关系数大于0.999;进样量2μL,仪器检出限为0.15 μg/L,定量限为0.5 μg/L.3个浓度下标准品的保留时间和峰面积相对标准偏差分别在0.03％～0.31％和0.41％～1.91％;玉米样品平均加标回收率为88.3％～96.7％.
A method was established for determining ochratoxin A in grain by high performance liquid chromatography.The linear detection ranges of ochratoxin A were 0.5-50μg/L with correlation coefficients of 0.999.The retention volume of ochra- toxin A was 2μL,the detection limit was 0.15μg/L,and the limit of quantification was 0.5μg/L.The relative standard deviations of retention time and peak area under three concentrations were between 0.03% to 0.31% and 0.41% to 1.9 l%.Recovery rates in corn spiked with ochratoxin A were between 88.3% to 96.7%.
出处
《粮食科技与经济》
2014年第4期31-32,56,共3页
Food Science And Technology And Economy
基金
北京市科学技术委员会项目"粮食及其制品中真菌毒素快速检测技术研究与应用"(Z121100008512001)
关键词
粮食
超高效液相色谱
Ochratoxin A, grain, High Performance Liquid Chromatography
