摘要
建立了检测啤酒中赭曲霉毒素A(OTA)免疫亲合柱净化高效液相色谱法。该方法为将脱气后的啤酒样品用15%NaCl和2%NaHCO_3的水溶液稀释,然后通过Ochra Test免疫亲和柱净化,采用C18(5μm,250×4.60 mm)色谱柱,流动相为乙腈-水-乙酸(体积比为99:99:2),流速0.900 mL/min;激发波长333 nm;发射波长477 nm;柱温35℃;进样量100μL;外标法定量。方法所采用的标准曲线有良好的线性关系,检出限为0.1 ng/mL,加标回收率为95%～106%,对不同浓度的样品相对标准偏差RSD为2.43%～8.32%。该方法操作简便、准确,回收率高,精密度良好,重现性好,可用于啤酒中赭曲霉毒素A的测定,具有实际应用价值。
Immunoaffinity Column clean-up and high performance liquid chromatography method （HPLC） for the determination of OTA in beer have been established. 20mL of degassed beer wete diluted with 4mL of a water solution containing 15%NaCl and 2% NaHCO3 ,then were cleaned up through OchraTest column. Separation was carried out on a column of C18 （5 μm, 250 × 4.60 ram）at 35℃ with acetonitrile-wateracetic acid（99 ： 99 ： 2, v/v） as mobile phase, elution flow rate 0.90mL/min. The detection of excitation wavelength was 333nm and the emission wavelength was 470nm, quantified by external standard method. The experimental results showed a good linear relationship between the peak area and the concentration, the recoveries for OTA were 95%～106%, and the relative standard deviation was 2.43%～8.32% for different kinds of beer. The results show that the method is simple, rapid, accurate and applicable for the determination of OTA in beer.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2007年第12期127-129,共3页
Food and Fermentation Industries
