摘要
目的建立复方氨酚美沙糖浆中盐酸甲基麻黄碱、愈创甘油醚、氢溴酸右美沙芬的定量分析方法。方法采用HPLC法测定,色谱柱为Agilent HC-C18（4.6 mm×250 mm,5μm）;流动相为甲醇—0.1%磷酸,梯度洗脱;流速为1 mL·min^-1,检测波长为216 nm。结果盐酸甲基麻黄碱、愈创甘油醚、氢溴酸右美沙芬分别在0.141 2-1.412、0.605-6.05、0.156 8-1.568μg范围内线性关系良好（r分别为0.999 6、0.999 8、0.999 5）;平均回收率分别为100.05%、98.69%、99.68%,RSD分别为2.24%、1.85%、2.03%（n=9）。结论该法快速准确,重复性好,可为复方氨酚美沙糖浆的质量控制提供定量评价方法。
Objective To establish a method for the quantitative determination of methylephedrine hydrochloride,guaifenesin and dextromethorphan hydrobromide in compound paracetamol and dextromethorphah syrup. Methods HPLC method was used and the separation was performed on Agilent HC-C18（4.6 mm ×250 mm,5 μm）column with gradient elution using methanol-0.1% phosphoric acid as mobile phase. The flow rate was 1 mL·min^-1and the detective wavelength was 216 nm. Results The linear ranges of methylephedrine hydrochloride,guaifenesin and dextromethorphan hydrobromide were 0. 141 2 - 1. 412 μg（ r = 0. 999 6）,0. 605 - 6. 05 μg（ r =0. 999 8） and 0. 156 8 - 1. 568 μg（ r = 0. 999 5） respectively. The average recoveries were 100. 05%,98. 69% and 99. 68 % with RSD 2. 24%,1. 85% and 2. 03%,respectively. Conclusions The method is rapid and accurate about determining methylephedrine hydrochloride,guaifenesin and dextromethorphan hydrobromide in compound paracetamol and dextromethorphah syrup.
出处
《安徽医药》
CAS
2014年第8期1437-1439,共3页
Anhui Medical and Pharmaceutical Journal
