摘要
探讨剔毒护肝方介导UEPG_2细胞凋亡的可能机制,方法:培养24h的HEPG_2细胞,分别给予剔毒护肝方(12.5mg/dl)及阿霉素(10μmol/ml)作用8d,同时设空白对照组,细胞凋亡采用流式细胞仪测定,Bcl-2、Bax、Fas采用S-P免疫组化法检测。结果:剔毒护肝方、阿霉素及空白对照组的凋亡率分别为24.43％、10.58％、2.05％。剔毒护肝方组细胞Bcl-2、Bax、Fas的表达量分别为0.118±0.015、0.152±0.028、0.121±0.018,与空白对照组及阿霉素组相比有显著性差异(P<0.01)。结论:剔毒护肝方有明显介导HEPG_2细胞凋亡的作用,并可能通过介导凋亡调控基因Pas、Pax的高表达及下调Bcl-2基因的水平来介导凋亡。
To study the probable mechanism of apoptosis of HEPG2 cell induced by Tidu Hugan Fang.
Methods: After HEPG2 cell line were planted for 24 hours, Tidu Hugan Fang and adriamycin (as the contrd group) were added, respectively to culture for 8 days. Meantime the normal group was set. Flow cytometry was used to analyse the rate of apoptosis in HEPG2 cell. S-P immunohistochemical method was used to identify the expression of Bcl-2、 Bax、 Fas gene. Results: The apoptosis rate of Tidu Hugan Fang, the control group and the normal group were 24. 43% , 10. 58% and 2. 05% , respectively. The expression of Bcl-2、 Bax、Fas in the Tidu Hugan Fang group were 0. 118±0. 015, 0. 152±0. 028 and 0. 121±0. 018 respectively. Compared with the control group and the normal group there were marking difference (P<0.001). Conclusion: Tidu Hugan Fang can obviously induce the apoptosis of HEPG2 cell, upregulate the expression of Fas and Bax gene and downregulate expression of Bcl-2 gene.
出处
《中西医结合肝病杂志》
CAS
2001年第4期220-221,共2页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
湖北省高等学校科学研究青年发展项目(No:98D024)
