摘要
目的分析使用胶体金免疫层析法(金标法)和酶联免疫法(ELISA)试验定性判断HIV抗体检测结果的可行性。方法以凉山州越西县全人群体检HIV快速检测阳性者为研究对象,分两组分别进行3次金标法和ELISA法检测,将检测至少1次阳性样本进行HIV抗体确证检测(免疫印迹法WB),分析确证结果与3次筛查结果一致性。结果共收集样本1 181例,金标法和ELISA法试验组分别为459例和722例,金标法检测3次均阳性样本数450例,其中449例(99. 78%) WB法为HIV-1抗体阳性,1例WB法为HIV-1抗体不确定,经随访为HIV抗体阴性,并且该样本金标法反应强度与HIV-1抗体阳性样本反应强度较接近; ELISA法检测3次均阳性样本数703例,其中701例(99. 72%) S/CO平均值≥6,且WB法为HIV-1抗体阳性。结论使用ELISA法检测HIV抗体时,结合检测阳性次数与S/CO平均值可定性判断HIV抗体检测结果。
Objective To analyze the feasibility of colloidal gold immunochromatography( GICA) and enzyme linked immunosorbent assays( ELISA) for the qualitative judgement of HIV antibody detection results.Methods HIV-positive people who were detected by a rapid test in the physical examination of the whole population in Yuexi County,Liangshan Prefecture were taken as the objects of this study. HIV-positive people were divided into two groups,and examined by GICA and ELISA for three times,respectively. People who had at least one positive sample were performed HIV antibody confirmatory test( western blotting). The consistency of confirmatory test results and three-time screening results was analyzed. Results A total of 1181 samples were collected,and there were 459 samples in GICA group and722 samples in ELISA group. About 450 samples were positive in all three-time GICA tests, of which 449 samples( 99. 78%) were HIV-1 positive antibody and 1 sample were indeterminate HIV-1 antibody by western blotting. The sample with indeterminate HIV-1 antibody was identified as HIV negative antibody through follow up,and the response intensity of this sample by GICA was close to that of HIV-1 positive samples. About 703 samples were positive in all three-time ELISA tests,of which 701 samples( 99. 72%) were with a mean value of S/CO ≥ 6 and with HIV-1 positive antibody by western blotting. Conclusion When HIV antibody was detected by ELISA,the combination of the number of positive results and the mean value of S/CO can qualitatively determine the test results of HIV antibody.
作者
袁丹
谭敏
金丹
汤捷
凌攀
袁风顺
周更生
刘红露
张灵麟
梁姝
YUAN Dan;TAN Min;JIN Dan;TANG Jie;LING Pan;YUAN Fengshun;ZHOU Gengsheng;LIU Honglu;ZHANG Linglin;LIANG Shu(Institute of STD/AIDS Control and Prevention,Sichuan Center for Disease Control and Prevention, Chengdu 610041,Sichuan Province,China;Yuexi County Center for Disease Control and Prevention,Liangshan Prefecture 616650,Sichuan Province,China.)
出处
《预防医学情报杂志》
CAS
2019年第3期228-230,共3页
Journal of Preventive Medicine Information
基金
四川省卫生厅2013年科研课题-四川省艾滋病新发感染监测及应用研究(项目编号:130003)
关键词
HIV
金标法
酶联免疫法
免疫印迹法
HIV
colloidal gold immunochromatography
enzyme linked immunosorbent assay
western blotting
