摘要
目的观察健脾益肾颗粒对Lewis肺癌小鼠免疫功能的影响。方法将80只C57BL/6J小鼠按随机数字表法分为4组:正常组、模型组、健脾益肾组和顺铂组,每组20只。除正常组外,其余各组小鼠右腋下皮下接种Lewis肺癌细胞。正常组不干预,模型组予磷酸盐缓冲液(PBS) 0. 2 m L/d灌胃,健脾益肾组予健脾益肾颗粒10 mg/(kg·d)灌胃,顺铂组予顺铂0. 5 mg/(kg·d)灌胃,均2天1次。持续2周后,取瘤重并计算抑瘤率;观察药物干预后肿瘤细胞的增殖能力、血中白细胞(WBC)、红细胞(RBC)、中性粒细胞(NEU)、血小板(Pt)、血红蛋白(HGB)及脾中NK细胞活性;检测IFN-γ和IL-10含量及T淋巴细胞比值。结果与正常组比较,模型组体重增量变低,RBC数量降低,WBC、NEU、Pt数量增加,NK细胞活性抑制,IFN-γ水平降低,IL-10水平升高,CD4^+T细胞数量CD4^+/CD8^+T比值降低(均P <0. 05)。与模型组比较,健脾益肾组和顺铂组小鼠体重增加,肿瘤重量降低,抑瘤率升高,肿瘤细胞数量于干预后第5天开始出现下降,且健脾益肾组体重增量、肺癌细胞数量干预优于顺铂组(均P <0. 05);健脾益肾组RBC数量升高,WBC、NEU以及Pt数量下降,NK细胞活性增加(均P <0. 05),IFN-γ水平升高,IL-10水平降低,CD4^+细胞数量和CD4^+/CD8^+比值增加(均P <0. 05)。结论健脾益肾颗粒对Lewis肺癌肿瘤具有免疫抑制作用。
Objective To observe the stimulative effect of Jianpi Yishen Granule on immunology of Lewis cancer mice model. Methods A Total of 80 C57 BL/6 J mice were randomly divided into 4 groups: normal group,model group,Jianpi Yishen( JPYS) group,and Cisplatin group,20 in each group. Mice in each group were inoculated with Lewis cancer cells,except for normal group,which did not receive any treatment. Model group received intragastric administration with0. 2 m L phosphate buffer solution( PBS) per day. The mice in JPYS group were treated with 10 mg/( kg·d) JPYS Granule,while Cisplatin group were treated with 0. 5 mg/( kg·d) every per twice day for 2 weeks. Mice were then sacrificed for the further examination of tumor weight and tumor inhibitory rate. MTT assay was performed to evaluate the tumor cell proliferation;serum red blood cell( RBC),white blood cell( WBC),neutrophil( NEU),and platelet( Pt),as well as splenic NK cell activity were detected;ELISA assay was utilized to examine the IFN-γ,IL-10,and T lymphocyte proportion. Results Compared with normal group,model groups displayed obvious weight loss,decreased RBC number,and increased WBC,NEU,Pt and HGB quantity. Meanwhile,NK cell activity in model group was significantly decreased. IFN-γ level was reduced whereas IL-10 increased. CD4^+T cell number and CD4^+/CD8^+ratio was decreased( all P < 0. 05). Compared with model group,mice in JPYS group and Cisplatin group showed significant weight gain,reduction of tumor weight and high tumor inhibitory rate,Lung cancer cell number began to decrease at day 5 after drug administration. JPYS group performed better than Cisplatin group in weight gain and tumor cell quantity intervene( all P < 0. 05). In JPYS group,RBC number was increased,while WBC,NEU,and Pt number were decreased. Meanwhile,NK cell activity in this group obviously increased.IFN-γ level was augmented while IL-10 was reduced. CD4^+T cell number and CD4^+/CD8^+ratio was significantly increased(P <0. 05). Conclusion JPYS Granule showed an immune-suppressing effect on Lewis lung cancer.
作者
陈晓波
郝世凯
王玉亮
武文杰
苏相相
姜爱娜
刘志霈
CHEN Xiao-bo;HAO Shi-kai;WANG Yu-liang;WU Wen-jie;SU Xiang-xiang;JIANG Aina;LIU Zhi-pei(Cell Storage Center,Vcanbio Cell & Gene Engineering Corp.,Ltd,Tianjin (300000);Department of Hematology,Tianjin First Center Hospital,Tianjin (300000);Institute of Hematology,Chinese Academy of Medical Sciences,Tianjin (300020);Vcanbio Cell & Gene Engineering Corp.,Ltd,Tianiin (300000))
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第2期237-241,共5页
Chinese Journal of Integrated Traditional and Western Medicine
