摘要
【背景】藻类是生产生物柴油的主要原料,而一些真菌和细菌能够与藻类共生并提高生物柴油产量,因此藻-菌共生培养技术成为国内外研究的热点。【目的】研究共生真菌Simplicilliumlanosoniveum对衣藻Chlamydomonas reinhardtii细胞生长和脂类合成的影响。【方法】将分离的蓝藻共生真菌和衣藻混合(共生)培养。【结果】与衣藻单独培养相比,混合培养衣藻的比生长速率(0.20 d-1)、细胞产率[0.17 g/(L·d)]和生物量(2.85 g/L)分别提高了10.3%、51.3%和55.7%;脂类比合成速率[0.68 mg/(g·d)]、合成速率[1.95 mg/(L·d)]和含量(220.4 mg/g)分别提高了33.3%、107.5%和32.0%,并且脂类中的饱和脂肪酸以及单不饱和脂肪酸C18-1和C18-2的比例上升,有利于生物柴油的加工。【结论】真菌Simplicilliumlanosoniveum能够促进衣藻的生长和脂类合成,因此藻-菌混合培养可用于生物柴油原料的生产。
[Background] Algae are the major feed-stocks for biofuel production,and some fungi and bacteria can establish symbiotic relationships with algae and promote their productivities.Hence,co-culture of algae with fungi or bacteria becomes the research hotspot worldwide.[Objective] To study the effects of cyanobacterium-symbiotic fungus Simplicillium lanosoniveum on the cell growth and lipid synthesis of Chlamydomonas reinhardtii.[Methods] C.reinhardtii was co-cultured with Simplicillium lanosoniveum.[Results] Compared to C.reinhardtii monoculture,the specific growth rate (0.20 d^-1 ),cell productivity (0.17 g/(L·d)) and biomass (2.85 g/L) of C.reinhardtii in co-culture increased 10.3%,51.3% and 55.7% respectively.The specific lipid synthetic rate (0.68 mg/(g·d)),lipid productivity (1.95 mg/(L·d)) and lipid content (220.4 mg/g) increased 33.3%,107.5% an 32.0% respectively.Also,the proportions of saturated fatty acids and monounsaturated fatty acids C18-1 and C18-2 in the lipid elevated,indicating the suitability for biofuel production.[Conclusion] Simplicillium lanosoniveum promotes cell growth and lipid synthesis of C.reinhardtii,and thus algae-fungi co-cultivation can be considered in biofuel feedstock production.
作者
董庆霖
王瑜琴
邢向英
郜海娇
李彤彤
DONG Qing-Lin;WANG Yu-Qin;XING Xiang-Ying;GAO Hai-Jiao;LI Tong-Tong(School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2018年第12期2639-2647,共9页
Microbiology China
基金
河北省自然科学基金(B2008000029)~~
关键词
共生真菌
衣藻
混合培养
细胞生长
脂类合成
Symbiotic fungus
Chlamydomonas reinhardtii
Co-culture
Cell growth
Lipid synthesis
