摘要
目的 :动态监测实验性脑出血大鼠血肿周边及下丘脑去甲肾上腺素 (NE)含量的变化规律并探讨其机制。方法 :采用胶原酶和肝素联合注入尾状核的方法建立大鼠脑出血模型 ,利用高效液相电化学法测定出血前及出血后 0 .5、6、12、2 4、48和 72小时各时间点血肿周边及下丘脑的 NE含量。结果 :大鼠脑出血后 30分钟血肿周边及下丘脑 NE含量开始同步升高 ,并于 2 4小时达峰值〔分别为 (5 5 5 .18± 2 0 .31) ng/ g和 (7846 .16±416 .12 ) ng/ g〕,48小时开始回落。结论 :大鼠脑出血过程中 ,脑内各部位去甲肾上腺素聚集 ,可使神经细胞过度兴奋 ,使局部脑血流进一步减少 ,并能影响神经内分泌功能 ,最终加重神经细胞损害。
Objective:To dynamically observe the changes in noradrenaline(NE) in central nervous system after experimental intracerebral hemorrhage(ICH) in rats.Methods:The ICH model was established by infusing collagenase and heparin into rat caudate nucleus.The changes in NE in perihemotoma brain tissue and hypothalamus were determined with highperformanceliquid chromatography before the experiment and at 0 5,6,12,24,48 and 72 hours after the establishment of ICH.Results:NE activity in perihemotoma brain tissue and hypothalamus increased simultaneously 0 5 hour after ICH,peaking at 24 hours,and began to reduce at 48 to 72 hours.Conclusions:Accumulation of NE in brain during cerebral hemorrhage may induce an excessive excitement in neural cells,reduce the regional cerebral blood flow,and affect the neuroendocrine function,thus aggravate the neural damage.
出处
《中国危重病急救医学》
CAS
CSCD
2002年第1期41-43,共3页
Chinese Critical Care Medicine
基金
重庆市医药卫生重点科研基金资助项目( No.2 0 0 12 0 0 3)
