摘要
为了建立食品中痕量铜的新分析方法，本文利用自行组装的激光诱导荧光－CCD二极管阵列检测器－计算机联用装置，研究了铜催化H2O2氧化罗丹明B的反应体系。在氨氟化铵(pH=9.0)缓冲溶液中，激发波长532.0 nm，发射波长580.0 nm条件下，采用催化荧光光度法实现了食品样品中痕量铜的测定。方法的线性范围0.5～4.0靏/L，方法的检出限为0.5 靏/L，加标回收率为86.9%～108.6％，相对标准偏差不大于9.70%。
For establishment of a new method for the determination of trace copper in food, the catalytic reaction of copper(II) for the oxidation of rhodamine B by hydrogen peroxide was studied by using the setup of laser induced fluorescence-charge coupled device diode array detector-computer. Determination of trace copper in food samples was completed in NH3-NH4F buffer solution (pH = 9.0) at lambda (ex) 532.0 nm and lambda (em) 580.0 nm by the catalytic spectrofluorimetric method. the linear range of the determination is 0.5 similar to 4.0 mug/L. The detection limit is 0.5 mug/L for copper. The Spiked recovery is in the range of 86.9% similar to 108.6% and the relatively standard deviation is not more than 9.7%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第9期1083-1085,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.39770646)
国家教委博士点基金(No.9760)资助课题
