摘要
为了检测食用油中邻苯二甲酸酯污染程度,建立食用油中邻苯二甲酸酯类的反相高效液相色谱测定方法。样品经正己烷溶解、弗罗里硅土固相萃取柱净化、氮气吹干、乙腈定容、高效液相色谱仪测定、外标法定量,在0.1～2.0 mg/L范围内具有良好的线性关系(r>0.99984),最低检测限小于50 ng/mL;在0.5μg/mL、1.0μg/mL和8.0μg/mL的添加水平,邻苯二甲酸酯的加标回收率在96%～104.4%之间,相对标准偏差在1.4%～2.8%之间;方法精密度实验的相对标准偏差在1.4%之内。结果表明:该方法简便、快速、稳定、灵敏、准确,适合用于对食用油中邻苯二甲酸酯的定性和定量测定。
In order to detect the pollution of phthalic acid ester in cooking oil,the method for determination of phthalic acid ester from cooking oil by RHPLC is presented.The sample is dissolved in hexane and purified by Florisil solid-phase extraction column.Then it is measured by HPLC,using external standard method for quantitative analysis.In the scope of 0.1-2.0 mg/L,it has good linear relationship（r0.99984）;lowest detectable limit smaller than 50 ng/mL;the recovery rate of the phthalic acid ester at 0.5 μg/mL,1.0 μg/mL and 8.0 μg/mL addition ranged from 96%-104.4%,the relative standard deviation was 1.4%-2.8%.The relative standard deviation of sample experimental precision was within 1.4%.The results show that this method is simple,rapid,stable,sensitive and accurate for qualitative and quantitative determination of phthalic acid ester in cooking oil.
出处
《西华大学学报（自然科学版）》
CAS
2011年第1期21-22,110,共3页
Journal of Xihua University:Natural Science Edition
基金
四川省食品生物技术重点实验室开放基金(SZJJ2009-013)
成都市科技局自然科学重点项目(10YTYB017SF-023)
关键词
邻苯二甲酸酯
反相高效液相色谱
二极管阵列检测
食用油
phthalic acid ester
reversed phase high performance liquid chromatography
diode array detector
edible oil
