摘要
目的 建立一个同时测定甘草甜素、甘草次酸血浆浓度变化监测的方法 ,进一步用于“脾主药动学”假说的验证 .方法 采用内标定量的反相高效液相色谱法 ,以甲醇 -水 -醋酸 (88∶ 11∶ 1)为流动相 ,ODS- 3柱 (15 0 mm× 4.6 mm,5μm)为固定相 ,在紫外 2 5 0 nm检测 .结果 GL,GA在此线性范围内 ,线性关系良好、精密度较高 .结论 本方法具有简便、灵敏、快速而准确的特点 ,适用于注射 GL制剂或口服中药复方前后 GL和 GA的体内、体外的药代动力学研究 。
AIM Establishment of method for measuring plasma concentration of the glycyrrhizin and glycyrrhetinic acid simultaneously by RP HPLC, in order to testify the hy pothesis of pharmacokinetics by traditional Chinese medicine. METHODS RP HPLC with internal labeling was employed. Methanol H 2O acetic acid (88∶11∶1) was used as mobile phase and ODS 3 (150 mm×4.6 mm, 5 μm) column as fixed phase, and detected by UV ( λ =250 nm). RESULTS Within the linear range the glycyrrhizin and glycyrrhetinic acid showed good linear relation and were detected with high precision.CONCLUSION Method is characterized by high simplicity, rapidity, and sensitivity, and is suitable for the in vitro and in vivo pharmacokinetic study of glycyrrhizin and glycyrrhetinic acid after oral administration or injection of GL extracts.
出处
《第四军医大学学报》
北大核心
2001年第12期1111-1113,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 !(3 9770 886
3 9670 865
3 9870 93 2
