摘要
建立了测定人血清中5-氟尿嘧啶的反相高效液相色谱法。采用ZorbaxODS柱4.6mm×250mm，预柱YWG-C184.6mm×50mm;甲醇:水（20:80）为流动相，紫外检测波长为273um，喃氟啶为内标定量。回收率为96.85％，田间和日内变异系数分别为537％和438％，血清中最低检测浓度为10μg／L。在0.04～50mg／L浓度范围内线性良好，相关系数为0.9990。
A reversed-phase HPLC method is described for determination of 5-fluorouracil in human serum. After being extracted with ethyl acetate 5-fluorouracil was analyzed by reversed-phase HPLC colunln (Zorbax ODS)and UV-detector (273nm)with tegaful as internal standard. The mobile phase was a mixture ot meLnyi alcohol and water(20: 80, V:V ) at a flow rate of lmL/min. The linear range was 0. 04-50mg/L(r = 0. 9990) and the detectable limit was 10ng/mL. The average recovery was 96. 85% and the coefficients of variation were 4. 38% and 5. 37 % for within-day and day-to-day tests respectively.
出处
《色谱》
CAS
CSCD
北大核心
1997年第2期164-165,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱法
氟尿嘧啶
血清
high performance liquid chromatography, 5-fluorouracil,human serum
