摘要
目的 研究 p38分裂原激活蛋白激酶 (MAPK)信号转导途径在胃癌耐药形成中的意义。方法 用免疫共沉淀法及Westernbolt法分别研究长春新碱处理胃癌细胞SGC790 1及胃癌多药耐药SGC790 1/VCR细胞内 p38MAPK活性及量的变化。 结果 胃癌多药耐药SGC790 1/VCR细胞及亲本SGC790 1细胞内均存在活性 p38MAPK ,长春新碱处理此两种细胞均可引起 p38MAPK活性增强 ,后者反应迅速 ,可在 2min内刺激p38MAPK活性增高 ,而前者反应较慢 ,至 30min时才观察到 p38MAPK活性增高。结论 p38MAPK信号转导途径可能与长春新碱的抗肿瘤活性相关 ,肿瘤细胞内
Objective To study the relation between p38 MAPK pathway and multidrug resistance of stomach cancer. Methods The activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR was examined by immunoprecipitation after treating with vincristine for 0,2,15,30,60 minutes. Results Vincristine could activate the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR. The difference between them was that in cell SGC7901 vincristine activate the p38 MAPK rapidly whereas in cell SGC7901/VCR slowly. Conclusion Vincristine could affect the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2001年第2期83-85,共3页
Chinese Journal of Digestion
基金
国家自然科学基金!(39470 785 )
全军医药卫生青年基金!(96Q0 78)
