摘要
为探索芸薹根肿菌的致病作用,采用水培法研究根肿菌毒素对油菜胚根和胚芽生长、幼苗致萎性、细胞膜透性、叶绿素含量、根系活力和幼苗生长的影响。结果表明:根肿菌毒素具有明显的生物活性,能够抑制油菜胚根和胚芽的生长,且随处理时间的延长和处理浓度的增加,幼苗萎蔫级数逐渐增加。毒素的作用可使油菜细胞膜透性增加,叶绿素含量和根系活力下降,其中叶绿素b的含量随处理时间的延长表现为先上升后下降的趋势,但仍低于对照。油菜幼苗的株高和主根长随毒素浓度的提高均呈逐渐下降的趋势,在85%和100%毒素浓度胁迫下,油菜幼苗株高及主根长均显著低于对照,说明植株的生长受到明显抑制。
Effects of Plasmodiophora brassicae toxin on the radicle and embryo growth, seedling wilting, cell membrane permeability, chlorophyll content, root activity and seedling growth of rape were studied to explore the pathogenic mechanism of P. brassicae Woron by water culture method. The results indicated that the toxin had significant biological activity, could inhibit the growth of radicle and embryo. The wilting degree of seedlings increased with extension of culturing time and toxin concentration. The rape under toxin exhibited an increase in membrane permeability but a decrease in chlorophyll content and root activity, especially chlorophyll b content firstly enhanced and then decreased with the increase of treatment time. With toxin concentration rising, seedling height and root length appeared gradually decreasing trend and dramatically lower than CK at 85% and 100% concentrations of toxin. It showed that the plant growth at this time was inhibited obviously.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第10期1-4,共4页
Guangdong Agricultural Sciences
基金
国家农业现代化四川创新团队-油菜病虫防治研究
关键词
芸薹根肿菌
毒素
油菜
生理特性
Plasmodiophora brassicae Woron
toxin
rape
physiological characteristics
