摘要
目的 研究白细胞介素１受体桔抗剂（ＩＬ－ｌｒａ）对ＩＬ－１β诱导人肾成纤维细胞（ＫＦＢ）增殖及产生纤连蛋白（ＦＮ）的影响。方法 体外培养ＫＦＢ，经ＩＬ-１β刺激后，分别采用～３Ｈ－ＴｄＲ掺入法和ＥＬＩＳＡ法测定 ＫＦＢ的增殖及其ＦＮ的分泌。结果 ＩＬ－１β（２５－１００ ｎｇ／ｍｌ）可促迸 ＫＦＢ增殖（Ｐ＜０．０５及分泌ｍ（Ｐ＜０．０５）； ＩＬ－ｌｒａ（１２５～１０００ｎｇ／ｍｌ）及ＩＬ－ｌｒａ（２５～１０００ｎｇ／ｍｌ 可以分别抑制ＩＬ－１β（５０ｎｇ／ｍｌ）引起的ＫＦＢ增殖（Ｐ＜０．０５）及ＦＮ分泌（Ｐ＜０．０５。结论 ＩＬ－１β在肾间质纤维化中起了一定的致病作用，ＩＬ－ｌｒａ可以阻断ＩＬ－１β的作用，从而为ＩＬ－ｌｒａ防治肾间质纤维化提供一定的实验依据。
Objective To study the effect of interleukin-1 receptor antagonist(IL-lre )on the proliferation and fibrolection(FN)) secretion of human kidnney fibroblasts(KFB) induced gy IL-1β. Methods The KFB were cultured and stimulated by lL-1β in vitro. The proliferation and FN secreting of KFB were detected by^3H-TdR incorporation and ELISA respectively. Results IL-1βP(25 ~ 100 ng/ml) could induce proliferation(P < 0. 05 ) and FN secretion(P < 0. 05 ) of KFB. IL--lra(125 ~ 1000 ng/ml) and IL-l ra (250 ~ 1 000 ng/ml) could suppress the effects of proliferation(P < 0. 05 ) and FN secreting(P < 0. 05 ) of KFB respectively induced by IL-1β(50 ng/ml). Conclusion IL-1β plays a role in renal interstitial fibrosis and IL-lra ean inhibit this effect of IL-1β. which provides a laboratory basis for clinical prevention and treatment of renal fibrosis.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2001年第1期23-26,共4页
Chinese Journal of Nephrology
基金
CMB基金资助项目(96-2-196)
