摘要
【目的】通过分析生脉注射液致RBL-2H3细胞与大鼠腹腔肥大细胞脱颗粒及其对2种细胞的细胞毒作用,评价生脉注射剂的致类过敏情况。【方法】分离大鼠腹腔肥大细胞,加入不同浓度的生脉注射剂共孵育,采用荧光法检测组胺并计算释放率。RBL-2H3细胞与不同浓度的生脉注射剂共孵育,采用显色法检测β-氨基己糖苷酶释放率。分别采用四甲基偶氮唑盐（MTT）法与流式细胞术检测生脉注射剂对2种细胞的细胞毒作用。【结果】生脉注射剂在终浓度为12.5～100μL/mL时可引起肥大细胞组胺释放率增加,25～100μL/mL时可引起RBL-2H3细胞β-氨基己糖苷酶释放率增加（均P〈0.05或P〈0.01）,上述剂量范围内对2种细胞均未表现细胞毒作用。【结论】生脉注射剂在未表现细胞毒作用的剂量下可引起大鼠腹腔肥大细胞和RBL-2H3细胞脱颗粒,提示生脉注射剂具有致类过敏的可能。
Objective The anaphylactoid action of Shengraai Injection （SI） was investigated by observing the degranulation of RBL-2H3 cells and rat peritoneal mast cells induced by SI as well as the cytotoxicity of SI to the two kinds of cells. Methods The rat peritoneal mast cells were isolated and incubated with different concentrations of SI, and the releasing rate of histamine was detected by fluorescence analysis. RBL-2H3 cells were cultured with different concentrations of SI and the releasing rate of β-hexosaminidase was assayed by coloration method. The cytotoxicity of SI to RBL-2H3 cells and rat abdominal mast cells was tested by methyl thiazolyl tetrazolium （MTT） assays and flow cytometry. Results SI at the concentration of 12.5-100 μL/mL could significantly induce the increase of histamine release in mast cells （P〈0.05） , and SI at the concentration of 25-100μL/mL significantly induced the increase of β-hexosaminidase release in RBL-2H3 cells （P〈0.05 or P〈0.01） Cytotoxicity of SI in the range of above concentration was not found. Conclusion SI below the cytotoxic dose shows certain effect on inducing the degranulation of rat peritoneal mast cells and RBL-2H3 cells, which indicates that SI may have the anat）hvlactoid action.
出处
《广州中医药大学学报》
CAS
北大核心
2014年第3期410-413,417,496,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东高校优秀青年创新人才培育项目(编号:LYM09049)
关键词
生脉注射剂/药理学
类过敏
脱颗粒
肥大细胞
细胞培养
Shengmai Injection/pharmacology
Anaphylactoid action
Degranulation
Mast cells
Cell culture
