摘要
目的研究金雀异黄素(genistein,Gen)对中波紫外线(UVB)照射人角质形成细胞系HaCaT细胞所致氧化损伤的保护作用。方法体外培养HaCaT细胞,分成空白对照组(A组),25μmol/L金雀异黄素处理组(B组),UVB(30mJ/cm2)辐射组(C组),UVB辐射(30mJ/cm2)+25μmol/L金雀异黄素处理组(D组),MTT法检测细胞增殖,流式细胞仪检测活性氧(ROS)水平,生物化学法测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果 D组与C组相比,细胞存活率明显增加(C组62%,D组85%),细胞内ROS水平明显降低(C组237.21,D组120.57,抑制率为49%),细胞内SOD和GSH-Px水平明显升高[SOD:C组(180.55±8.34)nU/mL,D组(233.42±6.52)nU/mL;GSH-Px:C组(310.72±22.52)U,D组(380.98±29.50)U],两组之间的差别均有显著的统计学意义。结论 Gen能增加细胞在UVB照射后的存活率,增加细胞内的抗氧化酶活性,降低细胞内ROS水平,对UVB照射所致人角质形成细胞氧化损伤具有保护作用。
Objective To investigate the effect of Genistein on HaCaT cells against oxidative damage caused by UVB. Methods HaCaT cells were cultured in vitro. Cells were divided into 4 groups. A group：blank control（without UVB and Genistein） ; B group： 25μM Genistein, without UVB; C group： 30mJ/cm^2 UVB, without Genistein ;D group ：30mJ/cm^2 UVB ,25 μM Genistein. MTT was used to detect the cell viability,flow cytometry was used to detect intracellular ROS production. SOD/GSH-px test kit was used to test intracellular GSH- Px,SOD activity. Results The cell viability was higher,the ROS production was lower,and the activities of GSH-Px, SOD were stronger in D group than those in C group. Cell viability：C group was 62% , D group was 85%. ROS：C group was 237.21, D group was 120.57, the inhibition ratio was 49%. SOD： C group was （ 180.55±8.34） nU/mL, D group was （233.42±6.52） nU/mL. GSH-Px ： C group was （310.72±22.52） U, D group was （ 380.98±29.50 ） U. There was statistically significant difference between the two groups. Conclusion Genistein could increase the cell viability after UVB irradiation, promote the activity of antioxidant enzyme, and reduce the intracellular ROS production. Genistein could protect HaCaT cells against oxidative damage caused by UVB.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2014年第4期349-351,354,共4页
The Chinese Journal of Dermatovenereology
基金
重庆市自然科学基金(CSTC.2010BB5021)
国家教育部留学归国基金
