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基于甲基乙烯基醚/马来酸酐共聚物水凝胶三维培养卵巢癌细胞的研究 被引量：2

Study of human ovarian cancer cells in three-dimensional culture based on poly( ethylene glycol)-cross linked poly( methyl vinyl ether-co-maleic acid) hydrogels
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摘要 目的：验证卵巢癌SKOV-3细胞在自制甲基乙烯基杉马来酸酐共聚物水凝胶中培养形成的3D模型。方法：通过SKOV-3细胞生长曲线、迁移和侵袭能力、耐药实验等，对比自制的水凝胶培养基与市售BME胶和胶原蛋白胶I胶培养基对SKOV-3细胞若干特性的影响。结果：SKOV-3细胞在自制水凝胶TZ028和TZ029培养基第10—15天时，为3D细胞培养最佳时期；在Transwell实验中表现出与胶原蛋白I胶相似的细胞侵袭力，并优于BME培养基；在紫杉醇耐药实验中形成细胞3D结构耐药性要高于2D细胞培养（P〈0．05）；与BME和胶原蛋白I胶培养基相比，该细胞对相同浓度下紫杉醇耐药性差异无统计学意义（P〉0．05）。结论：自制的甲基乙烯基彬马来酸共聚物和交联剂聚乙二醇交联反应形成的3D培养基质水凝胶，与商品化BME胶和胶原蛋白胶I胶培养基相比，具有类似的细胞培养功能。 Objective： To validate ovarian cancer cells cultured in three-dimensional（3D） culture based on poly （ethylene glycol）-cross linked poly （methyl vinyl ether-co-maleic acid ） hydrogels developed by our group. Methods： The abilities of proliferation, migration, invasion and resistant to paclitaxel of SKOV-3 cells were respectively evaluated in our developed poly（ methyl vinyl ether-co-maleic acid） （PMVE-co-MA）- based hydrogel compared to basal medium eagle（BME） and collagen I gel 3D culture. Results： The best growth period of SKOV- 3 cells in the developed hydrogel of TZ028 or TZ029 3D culture was 15-20 days. The result of Transwell assay shows that the invasion ability of SKOV-3 cells in the developed hydrogel 3D culture appeared similar result as in collagen I gel and better than BME 3D culture. The resistance to paclitaxel of SKOV-3 cells indicated that the formed 3D structure cells in the developed hydrogel of TZ028 or TZ029 exhibited high resistance compared with in 2D culture（ P 〈 0. 05 ）. There was no obvious different resistance to paclitaxel of SKOV-3 cells between the TZ028 or TZ029 3D culture and the BME or the collagen I gel 3D culture（P 〉 0. 05 ）. Conclusion： The developed poly （ethylene glycol）-cross linked poly （methyl vinyl ether-co-maleic acid） hydrogel 3 D culture has the similar cellular culture function as the commercialized BME and collagen I gel 3D culture.
作者 陈峻崧 陈登宇 窦骏 张天柱 杨翠萍 侍方方 顾宁
机构地区 东南大学医学院病原生物学与免疫学系 东南大学生物科学与医学工程学院 东南大学附属中大医院肿瘤科
出处 《东南大学学报（医学版）》 CAS 2014年第1期21-26,共6页 Journal of Southeast University(Medical Science Edition)
基金 国家科技部重大科学计划项目(2011CB933500)
关键词 三维培养 人卵巢癌 甲基乙烯基醚/马来酸酐共聚物水凝胶 化疗耐药 three- dimensional culture human ovarian cancer cell poly （ methyl vinyl ether- co- maleic acid）hydrogel chemoresistance
分类号 R737.31 [医药卫生—肿瘤] Q813.11 [生物学—生物工程]
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参考文献14

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共引文献16

同被引文献41

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东南大学学报（医学版）
2014年 第1期

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