摘要
目的探讨香连片对小鼠溃疡性结肠炎癌变过程中Wnt抑制因子1（WIF-1）和分泌型卷曲相关蛋白（SFRP-1）的影响。方法将60只BALB／C小鼠随机分为空白组（10只）、模型组（14只）、香连片低剂量组（12只）、香连片高剂量组（12只）和柳氮磺胺嘧啶组（12只）。除空白组外，其余各组采用DMH／DSS复合法诱导小鼠溃疡性结肠炎癌变模型，造模时间为18周。各组于造模前1周给予相应的干预措施，至造模的第10周结束；其中空白组和模型组予等量蒸馏水，各给药组给予相应的药物。用药时间为11周。观察小鼠体质量、粪便、耗食和精神状态等一般情况，结肠肿瘤的形成情况，HE染色镜下病变肠组织形态学改变，并以Real-timePCR检测WIF-1和SFRP-1mRNA的表达。结果在造模后期（第13-18周），模型组小鼠耗食减少，精神委靡，体质量明显减轻，与空白组比较，差异具有统计学意义（P〈0．01）；而香连片低剂量和高剂量组小鼠体质量下降与模型组比较无明显差异（P〉0．05）。模型组结肠黏膜可见团块状肿块形成，HE染色可见模型组小鼠结肠黏膜腺体增生明显、细胞排列紊乱、核染色加深；香连片低剂量、高剂量组虽可见腺体增生，但细胞分化未见异型性。香连片各剂量组WIF-1mRNA表达上调，与模型组比较，差异具有统计学意义（P〈0．05）；香连片各剂量组与模型组比较，SFRP-1mRNA表达差异无统计学意义（P〉0．05）。结论香连片IJ以通过减轻小鼠溃疡性结肠炎延缓肠黏膜细胞的不典型增生，其机制与上调WIF-1基因的表达，从而抑制Wnt／13-eatenin信号通路的异常激活有一定的关系。
Objective To investigate the effect of ＂Xianglian Tablet＂ on expression of WIF-1 and SFRP-ImRNA in mice with ulcerative colitis carcinogenesis. Methods Sixty BALB/C mice were randomly divided into five groups： blank group （ n = 10） , model group （ n = 14） , low dosage （ n = 12 ） and high dosage ＂ Xianglian Tablet＂ groups（ n = 12） , and Salazosulfadimidine group（ n = 12）. The models of carcinogenesis were induced by intraperitoneal injection of DMH and oral administration of DSS. The modeling time was 18 weeks, and medication for 10 weeks. The general changes of weighs, ieces, consumption of food and mental status were detected, and the formation of colon tumor and morphological changes of colon were detected. The expression of WIF-1 and SFRP-ImRNA were measured by Real-time PCR. Results From the 13th to 18th week after modeling, the consumption of food was less, the weight was reduced and their spirits were drooping in model group, with significant difference between blank group and model group （ P 〈0.01 ） , and the weight was reduced in low dosage and high dosage ＂ Xianglian Tablet＂ groups, without significant difference between ＂Xianglian Tablet＂ groups and blank group （ P 〉 O. 05 ）. The tumor masses were fouad in model group, anti the HE staining results indicated that the colon mucous gland byperplasia, cells arranged in disorder, nuclear staining deepen in model group ; although there was gland hyperplasia in ＂Xianglian Tablet＂ groups, no atypia was found in cell differentiation. ＂Xianglian Tablet＂ groups can up-regulate the expression of WIF- 1 mRNA, with significant difference from the model group （ P 〈 0.05 ） ; no significant difference was found in SFRP- 1 mRNA expression between ＂Xianglian Tablet＂ groups and model group （ P 〉 0.05 ）. Conclusion ＂Xianglian Tablet＂ can prevent the uk：erative colitis and retard the hyperplasia of mucosal cells. Its mechanism may be association with up-regulating the WIF-lmRNA expression and inhibiting the activation of Wnt/13-catenin signal transduction.
出处
《上海中医药杂志》
2014年第2期76-79,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市教委项目(2011JW07)
上海市教委重点学科建设项目(J50301)
关键词
香连片
溃疡性结肠炎癌变
Wnt抑制因子1基因
分泌型卷曲相关蛋白1基因
＂Xianglian Tablet＂
ulcerative colitis cancerization
Wnt inhibitol~ factor （ WIF- 1 ）
secreted frizzled-related protein （ SFRP- 1 ）
