摘要
本研究利用微卫星技术检测晋南牛保种场育种核心群遗传结构．建立其微卫星档案．从分子水平上为晋南牛的保种与选育提供理论依据。实验检测分析晋南牛16个微卫星位点的等位基因、基因频率及分布状况。结果表明：HEL9位点在150头晋南牛中呈单态．其他15个位点有效等位基因数在1～4．BM18180为低度多态（PIC〈0．25），BM2113、ETH、HEL5及TGLA44属于中度多态（0．5〉PIC〉0．25），其余位点为高度多态（PIC〉0．5），这些位点适用于动物的遗传分析和个体鉴定。结果显示，晋南牛保种群是一个具有遗传多样性的群体。
Genetic structure of Jinnan cattle preserved population was detected by microsatellite technology to set up microsatellite database and provide some theoretic basis of conservation and breeding. Alleles, frequencies and distribution of 16 microsatellites were analyzed. Results showed that marker HEL9 was monomorphic locus in 150 individuals, but effective number of alleles of the other 15 loci ranged 1-4. BM18180 showed low genetic polymorphisms （PIC〈0.25）, and microsatellites BM2113, ETH, HEL5 and TGLAdd22 were middle polymorphic （0.25〈 PIC〈0.5）, and the others were high （PIC〉0.5）, so that these microsatellite markers could be used for genetic diversity and individual identification. Jinnan cattle preserved population was a genetic diversity population.
出处
《中国畜牧杂志》
CAS
北大核心
2014年第3期22-26,共5页
Chinese Journal of Animal Science
基金
"十二五"国家高技术研究发展计划(863计划)子课题(2011AA100307-05)
动物营养学国家重点实验室开放课题(2004DA125184F1208)
山西省科技攻关项目(20130311024-1
201203 11021-1)
山西省农业科学院育种工程项目(11yzgc017)
关键词
微卫星
遗传多样性
晋南牛
microsatellites
polynorphism
Jinnan cattle
