摘要
目的观察不同剂量硫氢化钠(NaHS)的抗动脉粥样硬化(AS)效应,综合评价外源性硫化氢(H2S)的作用。方法健康雄性SD大鼠50只,随机平均分为对照组、模型组、5.6 mg/kg NaHS组、2.8 mg/kg NaHS组和1.4 mg/kg NaHS组。采用高脂饲料结合给予维生素D3(VitD3)的方法制备AS模型。喂养12周后比较各组动物体质量、血脂、血H2S水平和主动脉病理变化。结果 5.6 mg/kg NaHS组体质量较模型组明显下降(P<0.05),且有4只动物死亡。模型组与对照组比较血清总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)明显升高(P<0.01),高密度脂蛋白胆固醇(HDL-C)升高(P<0.05);5.6 mg/kg和1.4 mg/kg NaHS组血脂水平与模型组比较无变化,2.8 mg/kg NaHS组TC和LDL-C降低(P<0.05),但仍高于对照组(P<0.01)。模型组H2S水平较对照组明显下降(P<0.01);3个NaHS组血清H2S水平均显著高于模型组(P<0.01),5.6 mg/kg NaHS组和2.8 mg/kg NaHS组H2S水平与对照组比较明显增高(P<0.01,0.05),1.4 mg/kg NaHS组与对照组比较无差异。模型组、5.6 mg/kg和1.4 mg/kg NaHS组主动脉均可见AS斑块,2.8 mg/kg NaHS组内皮略有损伤,内膜增厚,中层平滑肌细胞轻度增生,未见AS斑块。结论适当补充外源性H2S可提高AS动物血清H2S水平,降低血脂,抑制AS斑块形成。
[ Objective] To evaluate the effect of exogenous Hydrogen Sulfide （H：,S） on Atheroselerosis （AS） rats through different doses of sodium hydrosulfide （NariS）. [ Methods ] Fifty healthy male SD rats were randomly divid- ed into five groups： control, AS model, 5.6 mg/kg NariS, 2.8 mg/kg NariS and 1.4 mg/kg NariS. A rat model of atherosclerosis was established by high fat diet and Vitamin D3. The body weight, blood lipid, blood H2S and aortic pathological changes were compared afterl2 weeks. [Results] The body weight of 5.6 mg/kg Naris group was sig- nificantly decreased than model, and there were four animals died in this group. The level of total Cholesterol （TC）, low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C） in model group were increased significantly （P 〈0.01, P 〈0.05） compared with control group. Compared with model g＇roup, lipid levels of 5.6 mg/kg group and 1.4 mg/kg group were no changes. The TC and LDL-C in 2.8 mg/kg group were decreased （P 〈 0.05） compared with model group, but still significant higher than control group （P 〈0.01）. The level of H：,S in three Naris groups were higher than model group （P 〈0.01, P 〈0.05）, which was decreased significantly compared with control group （P 〈0.01）. The aortic AS plaque can be seen in model group, 5.6 mg/kg NariS group and 1.4 mg/kg Naris group. The 2.8 mg/kg blaHS group had significant improvement than model group with no AS plaque forma-tion. [ Conclusions ] Proper supplementation of exogenous H2S can increase the serum level of H2S, decrease the level of blood lipids and inhibit the formation of plaque in AS model animal.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第27期1-5,共5页
China Journal of Modern Medicine
基金
江苏高校优势学科建设工程资助项目(No:JX10131801095)
关键词
动脉粥样硬化
硫化氢
硫氢化钠
毒性作用
atherosclerosis
hydrogen sulfide
sodium hydrosulfide
toxic effect
