摘要
目的探讨中药复方益糖康对3T3-L1前脂肪细胞分化过程中胰岛素样生长因子(IGF)-1R、PI3K、AKT、mTOR信号转导的影响。方法以生长抑制2 d的3T3-L1前脂肪细胞为研究对象,给予含益糖康的大鼠血清、LY294002预处理细胞,用Western印迹检测IGF-1R、PI3K、AKT、mTOR的蛋白表达。结果益糖康下调IGF-1R的蛋白表达,LY294002组与正常组比较,IGF-1R的蛋白表达没有差异(P>0.05);益糖康、LY294002均能下调PI3K、AKT、mTOR的蛋白表达。结论中药复方益糖康通过PI3K、AKT、mTOR通路来抑制脂肪分化过程中的脂滴聚集。
Objective To investigate the effect of Chinese herbal compound Yitangkang on IGF-1 R,PI3K,AKT,mTOR signal transduction in 3T3-L1 preadipocyte differentiation process.Methods 3T3-L1 preadipocytes after growth inhibition at 2 days were used.The cells were pretreated with rat serum contained with Yitangkang or LY294002,and the expressions of IGF-1 R,PI3K,AKT and mTOR protein were detected by Western blot.Results Expression of IGF-1R protein was decreased in Yitangkang group,there was no significant difference in the expression of IGF-1 R protein between LY294002 and the normal groups.Expressions PI3K,AKT,mTOR protein were decreased in both Yitangkang and LY294002 groups.Conclusions Yitangkang inhibits lipid droplets aggregation of fat cell during differentiation through PI3K,AKT,mTOR pathway.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2013年第21期5379-5381,共3页
Chinese Journal of Gerontology
基金
辽宁省博士启动基金项目(No.20111125)
