摘要
目的研究中药复方"益糖康"对"脾虚证"代谢综合征(MS)大鼠血清炎症因子的影响。方法将30只清洁级雄性SD大鼠随机分为2组,正常组10只,MS脾虚证型组20只。正常组喂养标准大鼠饲料及饮水,MS脾虚证型组喂饲甘蓝、猪油脂且游泳至耐力极限。4周后,饲喂高糖高脂膳食饲料。21周后,将MS脾虚证型组随机分为2组:MS脾虚证型组10只,继续饲喂高糖高脂膳食饲料并每天生理盐水灌胃;MS脾虚证型益糖康干预组10只,继续给予高糖高脂膳食饲料同时,每天以中药复方"益糖康"药液灌胃,0.44 g/d。继续喂养及药物干预持续6周后,经静脉窦抽取静脉血,分离血清,对血清中炎症因子IL-6和TNF-α进行检测。结果中药复方"益糖康"干预前,饲喂高糖高脂膳食饲料各组大鼠血清中IL-6和TNF-α水平明显升高;干预后MS脾虚证型益糖康干预组大鼠血清中IL-6和TNF-α水平明显降低,与MS脾虚证型组比较有明显差异,差别有统计学意义(P<0.05),与正常组比较无明显差异。结论中药复方"益糖康"对MS脾虚证型大鼠血清中炎症因子IL-6、TNF-α有降低作用。
This study was designed to observe the effect of Chinese medicine Yi Tang Kang on the serum inflammatory factors of rats with metabolic syndrome （MS） of spleen deficiency. Thirty male SD rats were randomlydivided into two groups： normal group, of which 10 rats were fed normal rodent diet and water, and MS group with spleen deficiency syndrome （MS-SDS）, of which 20 rats were fed cabbage and lard oil, and were then fed with thehigh sugar and fat diet 4 weeks later. After 21 weeks, the MS-SDS group was randomly subdivided into two groups： group Ⅰ, of which 10 rats were fed high sugar and fat diet and intragastrically perfused with saline everyday, andgroup Ⅱ, of which 10 rats were fed high sugar and fat diet and intragastrically perfused with Chinese medicine Yi Tang Kang everyday. After 6 weeks intervention, blood samples were collected from each rat via jugular vein andthe sera were prepared for IL-6 and TNF-α detection. Results showed that the IL-6 and TNF-α level in MS-SDS group were significantly higher than those in normal rats. However, the IL-6 and TNF-α protein levels in rats fromgroup Ⅱ that received Yi Tang Kang intervention were markedly lower than those in group Ⅰ that did not received drug intervention. Furthermore, the IL-6 and TNF-α levels in group I were comparable with those in normal group.These results indicated that Chinese medicine Yi Tang Kang can significantly decrease the levels of proinflammatory cytokines IL-6 and TNF-α in MS-SDS rat.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2013年第6期549-552,共4页
Immunological Journal
