摘要
目的:建立大栀子药材HPLC指纹图谱研究方法,并比较大栀子药材普通粉与超微粉指纹图谱的差异,以揭示超微粉碎对大栀子化学成分溶出的影响。方法:采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-0.4%磷酸为流动相进行梯度洗脱,流速1.0 mL/min,0～40 min检测波长为240 nm,40 min后为440 nm。结果:通过对10批大栀子药材指纹图谱的分析,确立了23个共有峰,10批大栀子药材的相似度评价结果均大于0.9;大栀子超微粉碎后,化学成分种类数目并未发生改变但其主要化学成分的含量明显提高。结论:该方法准确可靠,重现性好,可作为大栀子质量控制的有效方法。超微粉碎能促进大栀子化学成分的溶出。
Objective：To establish an HPLC fingerprint of Gardenia jasminoicles f. longicarpa and compare the differences between its ordinary powder and ultrafine powder. Methods ：The analysis was carried out on a Kromasil Cls （250 mm ×4.6mm,5μm）column with gradient elutiou of acetonitrile-0.4% phosphoric acid at the flow rate of 1.0 mL/min. The wavelength was 240 nm during 0 - 40 rain and 440 nm during 40 - 80 min. Results ： HPLC fingerprint of Gardenia jasminoides f. longicarpa was established, 23 common peaks were identified, and the similarity of 10 samples was greater than 0. 9. Ultrafine grinding did not change the types and number of chemical compositions, but it obviously increased the content of main chemical compositions. Conclusion ： The HPLC fingerprint is accurate,reliable and repeatable, which can be used for quality control of Gardenia jasminoides f. longicarpa. Uhrafine grinding can stimulate the release of chemical components of Gardenia jasminoides f. longicarpa.
出处
《中药材》
CAS
CSCD
北大核心
2013年第7期1072-1075,共4页
Journal of Chinese Medicinal Materials
基金
国家科技部支撑计划蒙药产业化共性关键技术研究项目(2012BAI28B01)
关键词
蒙药大栀子
超微粉
HPLC指纹图谱
Gardenia jasminoides Ellis f. longicarpa Z. M. Xie et M. Okada
Uhrafine powder
HPLC fingerprint
