摘要
目的:实验观察金边祛风饮对类风湿关节炎滑膜细胞IL-1β、TNF-α及MMP-3表达的影响。方法:类风湿关节炎及骨关节炎患者关节滑液经原代培养,应用2代传代的滑膜细胞,分为治疗组(RA)与对照组(OA)。用ELISA法检测金边祛风饮对类风湿关节炎患者滑膜细胞炎症因子IL-1β、TNF-α和MMP-3水平的表达,MTT法测定滑膜细胞增殖率。结果:不同浓度金边祛风饮刺激72小时后,滑膜细胞上清液TNF-α、IL-1β、MMP-3水平均降低,以高、中剂量最明显(P<0.05)。各剂量金边祛风饮均有抑制滑膜细胞生长的趋势(P<0.05),以高剂量最为明显(P<0.01),与对照组比较,有显著性差异(P<0.05)。结论:金边祛风饮能有效抑制类风湿关节炎滑膜细胞IL-1β、TNF-α及MMP-3表达。
Objective：To observe the influence of JBY liquid on production of IL-1β,TNF-α and MMP-3 in cultured synovial fibroblasts from patients with rheumatoid arthritis.Methods：The synovial fluid was collected from patients with rheumatoid arthritis and osteoarthritis to culture synovial cells divided into the treatment group（RA） and control group（OA）.The second generation synovial cells are stimulated with low,middle,high dose of JBY,and the level of proinflammatory cytokines IL-1β,TNF-α and metalloproteinase MMP-3 were detected by ELISA and RT-PCR.The proliferation of the cells was evaluated by MTT.Results：Stimulated 72 h with different concentration of JBY,the TNF-ɑ,IL-1β,MMP-3 of synovial cell supernatant were decreased,the high dose and middle dose were obviously（P0.05）.The growth of synovial cell was inhibited by different concentration of JBY（P0.05）,middle dose was obviously（P0.01）,compared with the control group,there was significant difference（P0.05）.Conclusion：JBY can effectively inhibit the TNF-ɑ,IL-1β,MMP-3 of rheumatoid arthritis synovial cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第9期952-954,959,共4页
Chinese Journal of Immunology
基金
湖北省教育厅课题项目(Z20082901)
湖北民族学院校园项目(MY2011B008)
关键词
金边祛风饮
滑膜细胞增殖
类风湿关节炎
炎性细胞因子
Jin Bian Qu Feng Yin（JBY）
Proliferation of synovial cell
Rheumatoid arthritis
Inflammatory cytokines
