摘要
目的该地区围产期妇女B群链球菌(GBS)感染情况及相关研究。方法围产期妇女和非妊娠妇女各500例,同时从阴道和直肠部位采样,用直接接种在血液培养基和经选择性肉汤培养基增菌后的方法进行GBS培养;凝集法做血清分型;K-B法进行药敏试验。对GBS感染潜在性影响因素(流产史、宫内节育器、妇科炎症)进行调查分析。结果 GBS共分离137株,其中围产期妇女72株(14.4%),非妊娠妇女65株(13.0%)。必须经由肉汤增菌后方可分离出GBS的有59株(5.9%),未发现仅从直接接种在血液培养基才分离出GBS的情形。阴道和直肠部位同时检出GBS的有46株(4.6%),仅阴道部位检出GBS的有71株(7.1%),仅直肠部位检出的有20株(2.0%)。GBS血清学分型主要为Ⅴ(38,27.7%)、Ⅲ(32,23.4%)、Ⅰa(22,16.1%)。药敏试验中,青霉素、氨苄西林的耐药率分别为5.8%、9.5%。在GBS感染潜在性影响因素中,妇科炎症患者与无妇科炎症者GBS检出率比较差异有统计学意义(P<0.01)。该地区围产期妇女与非妊娠妇女GBS感染比较差异无统计学意义(P>0.05)。结论改进培养方法可有效提高GBS筛检的阳性率;临床应重视GBS培养及药敏试验;菌株分型以Ⅴ、Ⅲ、Ⅰa为多见;妇科炎症与GBS感染关系密切相关。
Objective To study the perinatal Group B Streptococcus（GBS）infection and related conditions.Methods Perinatal women（500cases）and non-pregnant women（500cases）,sampling from vaginal and rectal specimens,with GBS were separated and checked by direct plating onto blood agar plates and the selective broth medium method（Lim broth）;Used agglutination method to test serotyping;Kirby-bauer agar diffusion method was used to judge the drug sensitivity.To investigate the potential impact factors of GBS infection（Abortion history,intrauterine device,gynecological inflammation）.Results The separated 137strains of GBS,including 72strains of perinatal women,65strains of non-pregnant women.There were 59（5.9%）positive GBS specimens recovered using LIM broth only and no positive GBS specimens recovered using direct plating onto blood agar plates only.46strains（4.6%） of GBS isolated from the vaginal and rectal specimens,only 71strains（7.1%）of GBS isolated from the vaginal specimens,only 20 strains（2.0%）of GBS isolated from the rectal specimens.GBS serotyping wereⅤ（38,27.7%）,Ⅲ（32,23.4%）,Ⅰa（22,16.1%）.Drug sensitivity test showed the resistance rates were penicillin（5.8%）and ampicillin（9.5%）.Potential impact factors for GBS infection,the gynecological inflammation group was significantly higher than the Non-gynecology inflammation group（P〈0.01）.Perinatal women compare with non-pregnant women shows no significant difference（P〈0.05）.Conclusion To use a more effective approach to screening for GBS carriage definitely enhance the detection rate.Clinicians should pay attention to GBS culture and drug sensitivity test.Serotyping shows mainly for V,Ⅲ,Ⅰa.Gynecological inflammation associated with GBS infection is closely.
出处
《国际检验医学杂志》
CAS
2013年第18期2370-2371,2373,共3页
International Journal of Laboratory Medicine
基金
佛山市顺德区人口和卫生药品监督局资助项目(2011094)
关键词
围生医学
链球菌感染
微生物敏感性试验
perinatology
streptococcal infections
microbial sensitivity tests
