摘要
为了测定宝乐果中游离氨基酸的种类以及含量。该文的样品用6-氨基喹啉-N-羟基琥珀酰亚胺基-氨基甲酸酯(AQC)柱前衍生后进行高效液相色谱(HPLC)分析。采用Phenomenex Luna C18(250 mm×4.6 mm,5μm)色谱柱,柱温为35℃;流动相A为去离子水,流动相B为乙腈,流动相C为10 mmol/L醋酸铵缓冲液(以冰醋酸调至pH 5.10),梯度洗脱,流速为0.8～1.0 mL/min;检测波长为248 nm,同时测定宝乐果中的游离氨基酸。结果得:18种氨基酸在0.02～0.5 mmol/L浓度范围内有良好的线性关系,r均在0.998 3～0.999 2之间;宝乐果中测得14种游离氨基酸,其含量范围为0.087～2.680 mg/g,平均加样回收率为96.9%～106.9%,RSD为0.67%～3.07%。该方法可用于检测宝乐果中游离氨基酸含量,测定的结果为宝乐果质量研究和评价提供了依据。
To determine and analyze free amino acids in Borojo. The sample was derivatized with 6-ami- noquinolyl-N-hydroxysuccinimidyl carbamate （AQC） and then was analyzed with high-performance liquid chromatography （HPLC）. HPLC was performed on a Phenomenex C18 （250mm x 4.6 mm, 5 p^m） col- umn with gradient elution of deionized water （ A）, acetonitrile （ B ） and 10 mmol/L ammonium acetate buffer solution （ adjusted to pH 5.10 with glacial acetic acid） （ C ） at the flow rate of 0. 8 - 1.0 mL/ min, detected with Ultra Voilet （UV） at 248 nm. Linear relationship equations were obtained with 18 kinds of amino acids in the range of 0. 02 - 0.5 mmol/L, and their correlation coefficients were in the range of 0. 998 3 ~ 0. 999 2. Fourteen amino acids were detected and average recoveries of those added were in the range of 96.9 % - 106.9 % and RSD values were in the range of 0.67 % - 3.07 %. The method could be used for determination of content of free amino acids in Borojo.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期100-104,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省科技现代生物技术资助项目(2011B010500014)
广州市科技天然活性物质开发利用研究资助项目(2011J4300077)
广东省科技项目农业攻关资助项目(2012A020602046)
