摘要
目的:对僵蚕抗惊厥活性部位分离鉴定的β-谷甾醇、麦角甾-6,22-二烯-3β,5α,8α-三醇及白僵菌素3个单体活性成分的抗惊厥活性筛选,为僵蚕质量标准提供体现中医药特色的内在指标成分。方法:根据僵蚕祛风定惊的功效,以苯巴比妥药物为阳性对照,5%吐温-80为空白对照,对分离得到的3个单体成分,以高剂量(250 mg·kg-1)和低剂量(125 mg·kg-1)连续3 d sc给药,于最后1次给药30 min后sc尼可刹米(750 mg·kg-1)及异烟肼(300 mg·kg-1)2种药物所致的动物惊厥进行抗惊厥活性筛选。结果:晶Ⅰ(β-谷甾醇)对2种惊厥模型惊厥潜伏期无明显作用;晶Ⅱ(麦角甾-6,22-二烯-3β,5α,8α-三醇)250 mg·kg-1对尼可刹米所致小鼠惊厥的出现时间均有延长作用,而其低剂量组对异烟肼所致小鼠死亡时间有加速作用;晶Ⅲ(白僵菌素)125 mg·kg-1组对尼可刹米所致小鼠惊厥的出现时间均有延长作用,而250 mg·kg-1组对异烟肼所致小鼠死亡时间有延长作用。结论:晶Ⅲ(白僵菌素)具有抗惊厥活性可作为僵蚕质量控制的指标性成分。
Objective：To screen anticonvulsive activity of compounds isolated from Beauveria bassiana.Method：Two anticonvulsive models indued by nikethamide（750 mg·kg-1,sc） and isoniazid（300 mg·kg-1,sc） were used to test the three compounds（β-sitosterol;ergosta-6,22-diene-3β,5α,8α-triol;beauvericin） isolated from B.bassiana.After 3 days of subcutaneous injection of the three compounds,nikethamide（750 mg·kg-1,sc） and isoniazid（300 mg·kg-1,sc） were injected after 30 minutes of the end administration.Result：Compound Ⅰ（β-sitosterol）,had no significantly effect on the two models;high-dose of compoundⅡ（ergosta-6,22-diene-3β,5α,8α-triol,250 mg·kg-1） prolonged the latent time of convulsions induced by nikethamide while low-level（beauvericin 125 mg·kg-1） increased the death time induced by isoniazid;low-level of compoundⅢ（beauvericin,125 mg·kg-1） prolonged the latent time of convulsions induced by nikethamide and high-level（250 mg·kg-1） prolonged the death time induced by isoniazid.Conclusion：CompoundⅢ（beauvericin） has anticovulsant activity,can be as a quality control index component of B.bassiana.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第17期248-250,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省中医药管理局重点资助项目(2003A07)
关键词
僵蚕
抗惊厥
白僵菌素
Beauveria bassiana
anticonvulsive
beauvericin
