摘要
目的运用超声提取法对裙带菜Undaria pinnatifida中岩藻黄素的提取工艺进行研究。方法以HPLC法检测岩藻黄素的量,通过单因素实验考察了提取溶剂、乙醇体积分数、提取时间、提取液料比、提取温度对岩藻黄素提取率的影响;并用正交试验得到超声波法提取岩藻黄素的最佳工艺。结果超声提取的最佳工艺是:乙醇体积分数为90%,超声时间为30min,提取温度为40℃,液料比10∶1。此条件下测得岩藻黄素的提取量为1.362mg/g。结论裙带菜中岩藻黄素的含量丰富,超声法提取岩藻黄素操作简单、方便快捷。
Objective To optimize the technology for the ultrasonic extraction of fucoxanthin from Undaria pinnatifida. Methods The concentration of fucoxanthin was analyzed by HPLC. Affecting factors for fucoxanthin extraction technique, such as solvent concentration, extraction temperature, extraction time, and ratio of sample weight to solvent volume were optimized by single factor and orthogonal test. Results The optimum extraction model is as follows： solvent concentration 90%, extraction temperature 40 ℃, extraction time 30 rain, and the ratio of sample weight to solvent volume 10：1. The fucoxanthin extraction rate was 1.362 mg/g. Conclusion U. pinnatifida is rich in fucoxanthin. The enhanced method of ultrasonic enhanced extraction is simple and accurate.
出处
《药物评价研究》
CAS
2013年第4期285-288,共4页
Drug Evaluation Research
关键词
岩藻黄素
裙带菜
超声提取
工艺优化
fucoxanthin
Undaria pinnatifida （Harv.） Suringar
ultrasonic extraction
technology optimization
