摘要
通过显微观察初步测定了不同浓度皂角苷(2.5、5、12.5、25、50、125 mg/L)融解大菱鲆红细胞的时间及其对白细胞的裂解作用;比较了4℃与20℃条件下,不同浓度皂角苷(2.5、5、12.5、25、50 mg/L)在体外对大菱鲆血细胞的溶血活性;用不同浓度的皂角苷溶液浸浴大菱鲆,得出皂角苷对大菱鲆的半致死浓度;同时分析了不同浓度皂角苷(0、5、25、45 mg/L)浸泡大菱鲆后血液中乳酸脱氢酶(LDH)活力变化。结果显示,显微观察实验中红细胞溶血时间与皂角苷浓度呈对数负相关(R2=0.982 5),皂角苷对大菱鲆白细胞也具有细胞裂解作用;50mg/L的皂角苷在20℃条件下5 min即可导致血细胞100%溶血,而4℃时处理相同时间仅为42.2%;皂角苷对大菱鲆的24 h半致死浓度(24h LC50)为64.85 mg/L。本研究的结果从细胞水平上初步评价了皂角苷的溶血毒性,为其安全使用提供了理论支持。
The correlation between hemolysis time and concentration of saponin （2.5,5,12.5,25,50 and 125 mg/L）was analyzed via microscope observation. In vitro hemolysis rate of turbot blood cells in different concentrations of saponin（2.5,5,12.5,25 and 50 mg/L） at 20 and 4 ℃, for different time duration were tested respectively. To investigate the toxicity of saponin extracted from QuiUaja saponaria to turbot （Scophthalmus maximus）, median lethal concentration （LC50）was calculated by immersing the turbot in sea water with different concentrations of saponin. In addition, the lactic dehydrogenase （LDH） activities in serum of turbot after bathing in saponin seawater solution （0,5,25 and 45 mg/L） were analyzed. The results indicated that the correlation between hemolysis time of erythrocytes and saponin concentration showed a logarithmic negative correlation（Rz = 0.98 ）, and that saponin could also induce hemolysis to leukocytes of turbot. Saponin in concentration of 50 mg/L could result in 100% hemolysis at 20 ℃ for 5 min,in contrast, only 42.2% hemolysis was observed at 4 ℃. The 24 h LC50 of saponin for S. maximus was 64.85 mg/L. In this report, from cell level, the evaluation of the toxicity of saponin to blood cells for hemolysis was made, which will theoretically support its safe application in aquaculture.
出处
《水产学报》
CAS
CSCD
北大核心
2013年第7期1073-1080,共8页
Journal of Fisheries of China
基金
国家自然科学基金项目(31172440)
关键词
大菱鲆
皂角苷
溶血
乳酸脱氢酶
Scophthalmus maximus
saponin
hemolysis
lactic dehydrogenase
