摘要
目的探讨不同品系小鼠瘤株跨系移植后移植瘤的生长转移情况，以及建立跨系小鼠肿瘤模型的可能性。方法不同品系来源的H22、S180、U14、FC、Ca761、SMG—A和DCS小鼠瘤株分别接种于C57BL／6J、ICR或KM小鼠皮下，观察小鼠体内成瘤和转移情况，绘制生长曲线。结果H22瘤株移植到C57BL／6J和ICR小鼠体内成瘤率均为100％。第17天时，C57BL／6J和ICR小鼠的瘤重分别为（2．8±O．4）g和（1．54-0．5）g，差异有统计学意义（P〈0．001）。S180瘤株在C57BL／6J小鼠体内的成瘤率为100％，肿瘤稳定生长。U14瘤株接种于C57BL／6J和KM小鼠后第32天，C57BL／6J小鼠体内移植瘤瘤重[（10．2．4-2．2）g]低于KM小鼠[（12．64-3．4）g，P=0．002]。接种U14瘤株的C57BL／6J和KM小鼠均出现肺和淋巴结转移。FC、Ca761和SMG-A瘤株接种于C57BL／6J小鼠后不能成瘤或成瘤后完全消退。DCS瘤株接种于C57BL／6J小鼠也有部分肿瘤发生消退，未消退的肿瘤在C57BL／6J小鼠体内连续传代，获得亚克隆DCS-C57瘤株，其在C57BL／6J小鼠稳定生长，成瘤率和肺转移率均为100％。结论小鼠瘤株跨系体内移植后，分化差、恶性程度高的瘤株呵成功建立移植瘤。免疫原性强的瘤株即使成瘤也会消退，无法跨系建立移植瘤。出现肿瘤部分消退的瘤株经连续传代后，可获得稳定的移植瘤模型。
Objective Mouse tumors were subcutaneously transplanted into different mouse strains and their growth and metastatic properties were checked, to explore the possibility of establishing animal tumor models in different mouse strains other than their normal host strains. Methods Seven mouse tumor ceil lines： I-I22, $180, U14, FC, Ca761, SMG-A and DCS were transplanted into C57BL/6J, ICR or KM mice, and their tumorigenicity, growth and metastasis were recorded and analyzed. Results The tumor formation rate of I-I22 cells in both the C57BL/6J and ICR mice was 100%, but the growth of H22 tumors was significantly faster in the C57BL/6J （2.8±0. 4）g than in the ICR mice （1. 5±0.5 ）g at the 17th day after transplantation （P 〈 0. 001 ）. The S180 tumors grew stably in C57BL/6J mice and the tumor formation rate was 100%. The U14 inoculated into C57BL/6J and KM mice showed both lymphatic and lung metastasis and formed significantly larger tumors in KM mice [ （12.6 ±3.4）g] than that in the C57BL/6J mice [ （ 10.2 ±2.2） g] on the 32rd day after transplantation （P = 0. 002）. Transplantation of FC, Ca761, and SMG-A did not form tumors or the tumors were completely regressed later in C57BL/6J mice. DCS cells formed tumors in C57BL/6J mice, but some of the tumors regressed. The retained tumors were passaged in C57BL/6J mice, and the substrain DCS-C57 cells was established which showed stable growth and had a 100% tumor formation rate and 100% lung metastasis rate in C57BL/6J mice. Conclusions Cross-strain transplanted tumors can be successfully established by inoculation of poorly differentiated and highly malignant tumor cells into different mouse strains. Some highly immunogenic tumor cells may form tumor, however, the tumors are regressed later, and can not establish cross-strain transplanted tumors in other mouse strains. Stable transplanted tumor models can be obtained from the partially regressed tumors after continuous passages in vivo.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2013年第7期486-490,共5页
Chinese Journal of Oncology
关键词
小鼠
细胞系
肿瘤
肿瘤消退
自行性
肿瘤转移
Mice
Cell line, tumor
Neoplasrm regression, spontaneous
Neoplasras metastasis
