摘要
目的:研究天花粉蛋白对小鼠黑色素移植瘤血管生成拟态、PI3K mRNA及蛋白表达的影响。方法:小鼠接种B16细胞复制模型,接种结束后小鼠随机分为天花粉蛋白高、中、低剂量组(0.6、0.4、0.2 mg/kg)和空白对照组接受治疗。观察肿瘤一般状况,CD31与过碘酸-雪夫双重染色法检测血管生成拟态形态学,RT-PCR检测PI3K mRNA的表达,免疫组织化学检测PI3K蛋白的表达。结果:与空白对照组相比,天花粉蛋白高剂量组(0.6 mg/kg)瘤组织体积与重量、VM密度、PI3K mRNA与蛋白的表达均明显降低。结论:天花粉蛋白能够抑制小鼠黑色素移植瘤的血管生成拟态,推测可能与调控PI3K的表达有关。
Object：To investigate the effects of trichosanthin on the vasculogenic mimicry and the expression of PI3K in the melanoma transplant tumour. Method： The mouse melanoma transplant tumor model was made with B16 melanoma cell suspending liquid. After vaccination the mice were treated with trichosanthin at 0. 6 mg/kg,0. 4 mg/kg,0. 2 mg/kg and normal saline. The weight and volume of tumor was observed, vasculogenic mimicry was detected by CD31-PAS double stain,the expression of PI3K was detected by RT-PCR and immuno-histochemistry. Results： Compared with the control group,the weight and volume of tumor,vasculogenic mimicry density,expression of PI3K in high dose group（0.6 mg/kg） was obviously decreased（P 0. 05） . Conclusion： Trichosanthin could inhibit vasculogenic mimicry in the melanoma transplant tumour,which we supposed may be related with the regulation to expression of PI3K.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第3期38-40,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
山东省中医药科技发展计划项目(2009-026)
山东省自然科学基金(ZR2010HQ008)
山东省高等学校科技计划(JIOLF13)共同资助
