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农杆菌介导大豆未成熟子叶节的遗传转化 被引量：1

A Preliminary Study on Agrobacterium-mediated Transformation of Soybean Immature Seeds Cotyledonary Node
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摘要 以冀豆7号未成熟子叶节为转化受体,研究外植体取材时间、低温预处理、共培养温度及光照时间对农杆菌介导大豆转化抗性芽的影响。结果表明,以开花后60～66 d进行外植体取材的子叶节产生的抗性芽数量较多;共培养温度26℃,光照时间24 h/d有利于抗性芽产生,而外植体4℃低温预处理不利于抗性芽产生。分析不同品种抗性芽诱导率发现,不同基因型未成熟子叶节抗性芽发生率不同,其中以豫豆22,冀豆7号和品系12的抗性芽诱导率较高。 Immature cotyledonary node of Jidou 7 were used as explants to research the impact tactors ot size after flowering 60～66 d. Resistant buds decreased when the explants were pretreated at 4 ℃ before infec- tion. The resistant buds rate reached to the highest under the condition of 26℃ and 24 h/d illumination in the co-culture stage. Also there was different in the resistant buds rate among varieties of soybean. In this study, the resistant buds rate of Yudou 22, Jidou 7 and Pinxi 12 were higher than jiunong 26, zheng 97196 and Pinxi 10.
作者 钟磊 王林红 乔亚科 崔姗姗 纪展波 李桂兰
机构地区 河北科技师范学院生命科技学院
出处 《河北科技师范学院学报》 CAS 2013年第1期1-4,共4页 Journal of Hebei Normal University of Science & Technology
基金 国家农业部转基因生物新品种培育重大专项(项目编号:2009ZX08004-001B 2009ZX08004-004B) 河北省自然科学基金项目(项目编号:C2009000868) 河北省科技支撑计划项目(项目编号:11220107D)
关键词 大豆 农杆菌介导 未成熟子叶节 抗性芽诱导 soybean transformation immature seed cotyledonary node
分类号 S565.103.52 [农业科学—作物学]
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参考文献15

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二级参考文献68

共引文献28

同被引文献18

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河北科技师范学院学报
2013年 第1期

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