摘要
目的:建立焦栀子配方颗粒中栀子苷的含量测定方法。方法:采用高效液相色谱法测定,色谱柱为WelchAQ-C18(4.6mm×250mm,5μm);以乙腈-水(15∶85)为流动相;检测波长为238nm;流速1.0ml/min。结果:栀子苷在0.1021～1.6340μg范围内,进样量与峰面积有良好的线性关系(r=1.0000,n=5);平均加样回收率为96.54%,RSD=1.14%(n=6)。结论:本方法简便、快速、稳定、准确可靠、重复性好,可用于焦栀子配方颗粒的质量控制。
Objective： To establish a method of determining the contents of geniposide in dispensing granules of car- bonized fructus gardeniae. Methods：HPLC was performed on Welch AQ-C18 column （4.6 minx250 mm,5~m） with acetonitrile-water （15：85）as mobile phase,detection wavelength of 238 nm and flow rate of 1.0 ml/min. Results： There was a good linear relationship （r=1.0000, n=5 ） between injection volume and peak area at the range of geni- poside 0.1021-1.6340 p,g, and the average recovery was 96.54%, RSD was 1.14%（n=6）. Conclusion：This method is simple ,rapid,stable,accurate and repeatable, so it can be used for quality control of dispensing granules of car- bonized fructus gardeniae.
出处
《广西中医药》
2013年第2期60-63,共4页
Guangxi Journal of Traditional Chinese Medicine
基金
广西千亿元产业重大科技攻关项目(桂科攻11107009-2)
关键词
焦栀子配方颗粒
栀子苷
HPLC
Dispensing granules of carbonized fructus gardeniae
geniposide
HPLC
