摘要
目的探讨环氧合酶-2(COX-2)基因多态性与结直肠腺瘤(CRA)易感性的关系。方法采用病例对照研究方法,应用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)和错配聚合酶链反应-限制性片段长度多态性技术(PIRA-PCR),分别检测110例CRA患者和120名健康人群的COX-2基因-765G>C、-1195G>A、8473T>C3个多态位点,采用χ2检验比较不同基因型与CRA易感性的关系。结果 CRA组和对照组的COX-2-765G>C、-1195G>A2个位点的3种基因型频率分布差异无统计学意义(P=0.972,P=0.313);两组间COX-2 8473T>C位点的3种基因型分布差异有统计学意义(P=0.034),Logistic回归分析显示,携带突变等位基因C的个体(8473TC+CC)CRA的易感性显著降低(OR=0.500,95%CI:0.274-0.913,P=0.024)。结论 COX-2-765G>C、-1195G>A可能与CRA易感性无关,COX-2 8473T>C与CRA易感性相关。
Objective To investigate the relationship between cyclooxygenase-2 （COX-2） single nucleotide polymorphisms （SNP） and the susceptibility of colorectal adenoma （CRA）. Methods We used polymerase chain reaction-restricted fragements length polymorphism （PCR-PFLP） and the primer-introduced restriction analysis （PIRA） PCR assay to genotype the COX-2 -765G〉C、-1195G〉A,8473T〉C polymorphisms in a case-control study of 110 CRA cases and 120 adenoma-free controls in Chinese population. Results The distribution of the genotype frequencies of -765G〉C and -1195G〉A were not statistically different between the cases and controls （P = 0. 972 for -765G〉C and P = 0. 313 for -1195G〉A）. Polymorphism of COX-2 8473T〉C were associated with the susceptibility to CRA. Logistic regression analyses revealed that the individuals with the 8473C allele had a decreased risk of CRA （OR = 0. 500, 95% CI： 0. 274-0. 913, P = 0. 024）. Conclusions Polymorphisms of COX-2 -765G〉C and-1195G〉A are not associated with the susceptibility to CRA. However, COX-2 8473T〉C can reduce the risk of CRA.
出处
《国际消化病杂志》
CAS
2013年第2期132-135,共4页
International Journal of Digestive Diseases
关键词
环氧合酶-2
基因多态性
结直肠腺瘤
Cyclooxygenase-2
Single nueleotide polymorphisms
Colorectal adenoma
