摘要
为研究海黍子硫酸多糖体外免疫与抗肿瘤活性,通过热水浸提法得海黍子多糖粗品(SKP),分别采用三氯乙酸法和透析法去除蛋白质和小分子杂质,再经DEAE-52离子交换柱层析纯化,分别由0.1、0.5、1mol/L NaCl洗脱得到3个多糖组分SKP1、SKP2、SKP3,对其总糖含量、硫酸基含量、糖醛酸含量、单糖组成、体外免疫活性及抗肿瘤活性进行分析。结果表明:SKP1总糖含量为72.7%,SKP2和SKP3总糖含量较高,分别为92.5%、94.7%;3种多糖糖醛酸含量差别不大,分别为17.24%、20.18%、20.33%;3种多糖主要由岩藻糖和半乳糖组成,还有少量木糖、甘露糖等,各种单糖比例不同;3种多糖均为硫酸酯多糖,硫酸基含量分别为3.4%、25.6%、29.9%;SKP2、SKP3均可显著增强小鼠细胞免疫功能,其中SKP2对脾淋巴细胞与腹腔巨噬细胞的增殖率最高可达53.56%和51.41%;SKP2对HT-29和HeLa细胞的抑制率最高可达57.4%和55.34%,SKP3为49.58%和50.86%,而SKP1仅为17.96%和13.59%,这种结果差异可能主要是由硫酸基含量不同引起的,总糖含量可以影响硫酸基含量从而间接对结果产生影响。
The in vitro immune-regulatory and anti-tumor activities of sulfated polysaccharides from Sargassum kjellmanianum （SKP） were investigated in this study. Polysaccharides were first extracted from Sargassum kjellmanianum by hot water. Crude extract was then treated with TCA to remove proteins and dialyzed to remove small molecule impurities. Three different types of polysaccharides, SKP1, SKP2 and SKP3, were collected through DEAE-52 ion exchange column. Chemical analysis showed that the polysaccharides were composed of fucose, galactose, xylose and mannose. All three types of polysaccharides were sulfated. Sulfate group accounted for 3.4%, 25.6% and 29.9% in SKP1, SKP2 and SKP3, respectively. SKP2 and SKP3 significantly enhanced immune function of immunocytes, and SKP2 increased the proliferation rate of spleen lymphocytes and peritoneal macrophages up to 53.56% and 51.41%. Cell culture showed that all SKP1, SKP2 and SKP3 inhibited the proliferation of HT-29 and HeLa cells, showing their anti-tumor activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第7期270-274,共5页
Food Science
基金
山东省自然科学基金项目(ZR2009BL03)
山东省优秀中青年科学家科研奖励基金项目(2008BS06012)
关键词
海黍子
硫酸多糖
体外免疫
抗肿瘤
Sargassum kjellmanianum
sulfated polysaccharides
in vitro immune
anti-tumorigenesis
