摘要
以云南萝芙木叶片为材料,通过RT-PCR方法首次克隆了云南萝芙木萜类吲哚生物碱(terpenoid indole alkaloids,TIAs)次生代谢的合成途径中重要的限速酶——异胡豆苷合成酶(strictosidine synthase,STR)的基因并进行了生物信息学分析。生物信息学分析表明,该基因的编码区长度为1 038 bp,编码345个氨基酸的多肽,等电点为5.06,N-端有一长度为27个氨基酸参与分泌的信号肽。二级结构预测指出,延伸链和不规则盘绕是STR蛋白质最大量的结构元件,而α-螺旋和β-转角则散布于整个蛋白质。同源性分析则表明,云南萝芙木中的STR和其它植物来源的STR同源,使用MEGA5.1构建了植物的STR分子系统进化树。
By using the leaf of Rauvolfia yunnanensis Tsiang as the material, utilizing RT-PCR, the strictosidine synthase gene,which was the key intermediate in terpenoid indole alkaloids （TIAs） was cloned and its bio-information was analyzed. The analysis results indicate that the DNA has been sequenced,revealing an open reading frame of 1038 base pairs, encoding 345 amino acids,an isoelectric point of 5.06, and 27 amino acids constructed a signal peptide that attend excrete at its N-end.The prediction of its secondary structure shows that extend strand and random coil was the most quantities in STR protein and a-spiral and β-outer corner distributed in the whole protein.The homology analysis manifests that STR in Rauvolfia yunnanensis Tsiang was homologous with STRs from other plant sources. MEGA5.1 was adopted to construct the phylogenetic tree of the STR.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2012年第6期128-131,共4页
Journal of Central South University of Forestry & Technology
基金
云南2007年度产业化重大关键技术开发项目[云发改高科(2007)1718号]
