摘要
[目的]从代谢组学角度研究系统性红斑狼疮(systemic lupus erythematosus,SLE)模型小鼠MRL/1pr与昆明种对照小鼠C57BL/6J尿液中代谢物差异,寻找代谢标记物,以探讨SLE的发病机制。[方法]应用高效液相色谱-四级杆-飞行时间质谱联用系统(HPLC-Q-TOF/MS),对SLE模型小鼠和对照组小鼠的尿液样本进行代谢图谱分析。应用Mass Hunter提取色谱峰信息,MPP(Mass Profiler Professional)软件筛选处理数据,数据模式识别方法采用有监督模式的偏最小二乘法(Partial Least Squares,PLS)。[结果]SLE模型小鼠和对照组小鼠代谢特征存在明显差异。根据PLS分析的载荷图和VIP值,进一步筛选对数据分型具有显著影响的离子,鉴别得到9个代谢标记物。[结论]对照代谢路径发现,氨基酸代谢异常是造成组间代谢差异的主要原因,这为从代谢途径研究SLE小鼠发病机制提供了实验依据。
[Objective] To find out metabolic markers through the metabolic differences between systemic lupus erythematosus（SLE） mice model（MRL/1pr） and control group（C57BL/6J） and investigate the pathogenesis of SLE.[Methods] A high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry method（HPLC-Q-TOF/MS） was used to analyze metabolic characteristic of the systemic lupus erythematosus mice and control group.Software of Mass Hunter was applied to extract the peak information.MPP（Mass Profiler Professional） was used to filter and process the metabolic data.Data pattern was recognized by using a supervised mode of Partial Least Squares（PLS）.[Results] It was not difficult to find that there were obvious differences between the SLE mice and C57BL/6J mice.Loading plot and variable importance in the projection（VIP） of PLS were used to search for the ions which had a significant contribution to classification,and the nine of these ions were identified.[Conclusion] The amino acid metabolic abnormalities are the main reasons for the metabolic differences between the groups.According to the experiment,it provides a metabonomics path to study the pathogenesis of SLE.
出处
《浙江中医药大学学报》
CAS
2012年第12期1325-1329,1332,共6页
Journal of Zhejiang Chinese Medical University
基金
高等学校博士学科点专项科研基金资助课题(20093322120001)
国家自然科学基金资助项目(81001515)~~
关键词
SLE
代谢组学
液相色谱-四级杆-飞行时间质谱
PLS
SLE
metabonomics
high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry method（HPLC-QTOF/MS）
PLS
