摘要
目的应用电喷雾离子阱质谱对高喜树碱类衍生物10-羟基-7-甲基-高喜树碱(TOP-01)进行质谱裂解分析,通过主要特征碎片离子研究其裂解规律。方法样品溶液通过仪器自带的蠕动泵进样系统直接进样分析,采用离子阱质谱的多级离子碰撞裂解方式,寻找TOP-01的特征碎片离子。结果 TOP-01在软电离模式下,主要产生的正离子碎片分别为m/z333、305、277、290等,负离子碎片分别为m/z 347、319、317、289、301等。结论 TOP-01在软电离模式下、正负离子模式下质谱裂解方式存在差异,其中主要产生的正负离子碎片分别为333和347,本研究为高喜树碱及其衍生物的结构修饰及代谢研究等提供了参考依据。
Objective To analyze the fragments of 10-hydroxy-7-methyl- homocamptotheein （ TOP-01 ） by eleetrospray ion trap mass spectrometry and deduce the MS/MS fragment pathway of it. Methods Samples were directly injected into the instrument and the fragments of the samples were yielded by using multi-stage ion trap mass spectrometry. Results Fragment ions m/z 333, 305, 277, 290 were generated at the positive ion mode and m/z 347, 319, 317, 289, 301 at the negative mode for TOP-01. Conclu- sion Differences existed in fragmentation pathway between positive and negative ESI-MS,and the main fi＇agmentation characteristics of TOP-01 were m/z 333 and 347 in positive and negative mode respectively. These results provided very important iutormation for the structural modification and the drug metabolic research of homocamptotheein.
出处
《药学实践杂志》
CAS
2013年第1期24-26,60,共4页
Journal of Pharmaceutical Practice
关键词
高喜树碱衍生物
10-羟基-7-甲基-高喜树碱
电喷雾离子阱质谱
结构解析
homocamptothecin derivatives
10-hydroxy-7-methyl-homocamptotheein
eleetrospray ion trap mass spectrome- try
structure elucidation
