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血清对脐带间充质干细胞体外培养的影响 被引量：3

Influence of serum on umbilical cord mesenchymal stem cells in vitro culture
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摘要 目的探讨血清对脐带间充质干细胞体外培养的影响。方法将人脐带间充质干细胞分别培养在不含血清、含灭活血清和含未灭活血清的低糖达尔伯克改良伊格尔培养基(L-DMEM)中。每天用显微镜观察脐带间充质干细胞在3种培养基中的生长情况,并计算细胞总数、统计细胞成活率。结果脐带间充质干细胞在含未灭活血清的L-DMEM中生长状况最好,在不含血清的L-DMEM中生长状况最差。结论含未灭活血清的L-DMEM是脐带间充质干细胞体外培养的最佳培养基。 Objective To explore the influence of serum on umbilical cord mesenchymal stem cells in vitro culture.Methods Human umbilical cord mesenchymal stem cells were cultured in low glucose-Dulbecco′s modified Eagle′s medium（L-DMEM） without serum,plus the inactivated serum and activated serum,separately.Umbilical cord mesenchymal stem cells in these three mediums were observed with a microscope every day,each cell number was calculated,and cell survival rate was statisticsed.Results Umbilical cord mesenchymal stem cell growth was best in L-DMEM which plus the activated serum,and worst in which without serum.Conclusion L-DMEM plus the activated serum is the best medium for the umbilical cord mesenchymal stem cell culture in vitro.
作者 邢雪琨 武红艳
机构地区 新乡医学院生命科学技术学院 新乡医学院基础医学院
出处 《新乡医学院学报》 CAS 2012年第12期896-899,共4页 Journal of Xinxiang Medical University
关键词 脐带间充质干细胞 胎牛血清 细胞总数 细胞成活率 umbilical cord mesenchymal stem cells fetal bovine serum cell number cell survival rate
分类号 Q28 [生物学—细胞生物学]
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参考文献10

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共引文献31

同被引文献49

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引证文献3

二级引证文献7

新乡医学院学报
2012年 第12期

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